Simultaneous determination of five neurotransmitters in neonatal rat hippocampus by adding vitamin C coupled with isotope dilution-ultra-high performance liquid chromatography-tandem mass spectrometry
Analysis of neurotransmitters in brain tissue is useful for mechanistic studies of the central nervous system. Isotope dilution coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the simultaneous determination of glutamic acid, γ-amino butyric acid, acetylcholine, dopamine, and serotonin in the hippocampus tissue. A 2% (v/v) acetic acid in water-methanol (9:1, v/v) solution was used to prepare the standards and re-dissolute samples after nitrogen drying. An Ultimate AQ-C18 column (150 mm×4.6 mm, 3 μm) was used as the analytical column and 0.1% (v/v) formic acid in water and methanol were used as the mobile phase. Gradient elution was performed and all target compounds were eluted over 3 min at 28℃. Vitamin C spiked during the sample pretreatment and storage periods significantly retarded the oxidation of dopamine and serotonin and improved the neurotransmitter stability. The developed method showed good linearities (correlation coefficient (R2)>0.998), low detection limits (0.15-1.0 μg/L), good inter-and intra-day precisions (relative standard deviations:0.39%-13.6%), good accuracy (92.9%-119%), low carry-over, and excellent stability. Moreover, the method was successfully applied and validated in the determination of rat hippocampus exposed to bisphenol A.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
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| Enthalten in: |
Se pu = Chinese journal of chromatography - 37(2019), 4 vom: 08. Apr., Seite 404-411 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Kuang, Hongxuan [VerfasserIn] |
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| Links: |
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| Themen: |
Ascorbic Acid |
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| Anmerkungen: |
Date Completed 31.07.2019 Date Revised 31.07.2019 published: Print Citation Status MEDLINE |
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| doi: |
10.3724/SP.J.1123.2018.11012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM295983205 |
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| 245 | 1 | 0 | |a Simultaneous determination of five neurotransmitters in neonatal rat hippocampus by adding vitamin C coupled with isotope dilution-ultra-high performance liquid chromatography-tandem mass spectrometry |
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| 520 | |a Analysis of neurotransmitters in brain tissue is useful for mechanistic studies of the central nervous system. Isotope dilution coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the simultaneous determination of glutamic acid, γ-amino butyric acid, acetylcholine, dopamine, and serotonin in the hippocampus tissue. A 2% (v/v) acetic acid in water-methanol (9:1, v/v) solution was used to prepare the standards and re-dissolute samples after nitrogen drying. An Ultimate AQ-C18 column (150 mm×4.6 mm, 3 μm) was used as the analytical column and 0.1% (v/v) formic acid in water and methanol were used as the mobile phase. Gradient elution was performed and all target compounds were eluted over 3 min at 28℃. Vitamin C spiked during the sample pretreatment and storage periods significantly retarded the oxidation of dopamine and serotonin and improved the neurotransmitter stability. The developed method showed good linearities (correlation coefficient (R2)>0.998), low detection limits (0.15-1.0 μg/L), good inter-and intra-day precisions (relative standard deviations:0.39%-13.6%), good accuracy (92.9%-119%), low carry-over, and excellent stability. Moreover, the method was successfully applied and validated in the determination of rat hippocampus exposed to bisphenol A | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a hippocampus | |
| 650 | 4 | |a neurotransmitters | |
| 650 | 4 | |a ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) | |
| 650 | 4 | |a vitamin C | |
| 650 | 7 | |a Isotopes |2 NLM | |
| 650 | 7 | |a Neurotransmitter Agents |2 NLM | |
| 650 | 7 | |a Ascorbic Acid |2 NLM | |
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| 700 | 1 | |a Tan, Jianhua |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Yifan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Shuhua |e verfasserin |4 aut | |
| 700 | 1 | |a Pang, Qihua |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Chuhua |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Ruifang |e verfasserin |4 aut | |
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