A Label-free aptasensor based on Aptamer/NH2 Janus particles for ultrasensitive electrochemical detection of Ochratoxin A
Copyright © 2019 Elsevier B.V. All rights reserved..
In this study, a novel aptasensor based on Aptamer/NH2 Janus particles is developed for the detection of Ochratoxin A(OTA). By coating gold on the hemispherical surface of the aminated polystyrene particles, Ochratoxin A aptamer is immobilized on the surface of the gold layer for selective identification and the other hemispherical able to bind to Glassy carbon electrode via peptide bond. Under optimum conditions, the sensor exhibited a wide dynamic range of OTA concentration from 1 × 10-5 nM to 10 nM, and the detection limit is 3.3 × 10-3 pM on condition of acceptable stability and reproducibility. The sensors were showed excellent performance in the detection of OTA in red wine sample with recoveries between 95.7% and 100.18%, which studied by the standard addition spiking technique. This work provides a new idea and method for preparing immune electrochemical sensors and is expected to be used for the OTA detection in red wine sample analysis.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:199 |
|---|---|
| Enthalten in: |
Talanta - 199(2019) vom: 01. Juli, Seite 310-316 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yang, Ya-Juan [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
1779SX6LUY |
|---|
| Anmerkungen: |
Date Completed 20.05.2019 Date Revised 20.05.2019 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.talanta.2019.02.015 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM295738502 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM295738502 | ||
| 003 | DE-627 | ||
| 005 | 20231225084316.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.talanta.2019.02.015 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0985.xml |
| 035 | |a (DE-627)NLM295738502 | ||
| 035 | |a (NLM)30952263 | ||
| 035 | |a (PII)S0039-9140(19)30150-X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Yang, Ya-Juan |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A Label-free aptasensor based on Aptamer/NH2 Janus particles for ultrasensitive electrochemical detection of Ochratoxin A |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.05.2019 | ||
| 500 | |a Date Revised 20.05.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019 Elsevier B.V. All rights reserved. | ||
| 520 | |a In this study, a novel aptasensor based on Aptamer/NH2 Janus particles is developed for the detection of Ochratoxin A(OTA). By coating gold on the hemispherical surface of the aminated polystyrene particles, Ochratoxin A aptamer is immobilized on the surface of the gold layer for selective identification and the other hemispherical able to bind to Glassy carbon electrode via peptide bond. Under optimum conditions, the sensor exhibited a wide dynamic range of OTA concentration from 1 × 10-5 nM to 10 nM, and the detection limit is 3.3 × 10-3 pM on condition of acceptable stability and reproducibility. The sensors were showed excellent performance in the detection of OTA in red wine sample with recoveries between 95.7% and 100.18%, which studied by the standard addition spiking technique. This work provides a new idea and method for preparing immune electrochemical sensors and is expected to be used for the OTA detection in red wine sample analysis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Electrochemical aptasensor | |
| 650 | 4 | |a Janus Particles | |
| 650 | 4 | |a OTA | |
| 650 | 7 | |a Aptamers, Nucleotide |2 NLM | |
| 650 | 7 | |a Ochratoxins |2 NLM | |
| 650 | 7 | |a ochratoxin A |2 NLM | |
| 650 | 7 | |a 1779SX6LUY |2 NLM | |
| 700 | 1 | |a Zhou, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Xing, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Guo-Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Cai-Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Lei, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Chuan |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Xu |e verfasserin |4 aut | |
| 700 | 1 | |a He, Yujian |e verfasserin |4 aut | |
| 700 | 1 | |a Shuang, Shao-Min |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Talanta |d 1966 |g 199(2019) vom: 01. Juli, Seite 310-316 |w (DE-627)NLM114409137 |x 1873-3573 |7 nnns |
| 773 | 1 | 8 | |g volume:199 |g year:2019 |g day:01 |g month:07 |g pages:310-316 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.talanta.2019.02.015 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 199 |j 2019 |b 01 |c 07 |h 310-316 | ||