SARS coronavirus protein nsp1 disrupts localization of Nup93 from the nuclear pore complex
Severe acute respiratory syndrome coronavirus nonstructural protein 1 (nsp1) is a key factor in virus-induced down-regulation of host gene expression. In infected cells, nsp1 engages in a multipronged mechanism to inhibit host gene expression by binding to the 40S ribosome to block the assembly of translationally competent ribosome, and then inducing endonucleolytic cleavage and the degradation of host mRNAs. Here, we report a previously undetected mechanism by which nsp1 exploits the nuclear pore complex and disrupts the nuclear-cytoplasmic transport of biomolecules. We identified members of the nuclear pore complex from the nsp1-associated protein assembly and found that the expression of nsp1 in HEK cells disrupts Nup93 localization around the nuclear envelope without triggering proteolytic degradation, while the nuclear lamina remains unperturbed. Consistent with its role in host shutoff, nsp1 alters the nuclear-cytoplasmic distribution of an RNA binding protein, nucleolin. Our results suggest that nsp1, alone, can regulate multiple steps of gene expression including nuclear-cytoplasmic transport.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2019 |
---|---|
Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:97 |
---|---|
Enthalten in: |
Biochemistry and cell biology = Biochimie et biologie cellulaire - 97(2019), 6 vom: 19. Dez., Seite 758-766 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Gomez, Garret N [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 26.03.2020 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1139/bcb-2018-0394 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM29565158X |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM29565158X | ||
003 | DE-627 | ||
005 | 20231227130009.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2019 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1139/bcb-2018-0394 |2 doi | |
028 | 5 | 2 | |a pubmed24n1224.xml |
035 | |a (DE-627)NLM29565158X | ||
035 | |a (NLM)30943371 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Gomez, Garret N |e verfasserin |4 aut | |
245 | 1 | 0 | |a SARS coronavirus protein nsp1 disrupts localization of Nup93 from the nuclear pore complex |
264 | 1 | |c 2019 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 26.03.2020 | ||
500 | |a Date Revised 13.12.2023 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Severe acute respiratory syndrome coronavirus nonstructural protein 1 (nsp1) is a key factor in virus-induced down-regulation of host gene expression. In infected cells, nsp1 engages in a multipronged mechanism to inhibit host gene expression by binding to the 40S ribosome to block the assembly of translationally competent ribosome, and then inducing endonucleolytic cleavage and the degradation of host mRNAs. Here, we report a previously undetected mechanism by which nsp1 exploits the nuclear pore complex and disrupts the nuclear-cytoplasmic transport of biomolecules. We identified members of the nuclear pore complex from the nsp1-associated protein assembly and found that the expression of nsp1 in HEK cells disrupts Nup93 localization around the nuclear envelope without triggering proteolytic degradation, while the nuclear lamina remains unperturbed. Consistent with its role in host shutoff, nsp1 alters the nuclear-cytoplasmic distribution of an RNA binding protein, nucleolin. Our results suggest that nsp1, alone, can regulate multiple steps of gene expression including nuclear-cytoplasmic transport | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, N.I.H., Extramural | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a NLS | |
650 | 4 | |a Nup93 | |
650 | 4 | |a SARS-CoV | |
650 | 4 | |a SLN | |
650 | 4 | |a SRAS-CoV | |
650 | 4 | |a complexe des pores nucléaires | |
650 | 4 | |a immunofluorescence | |
650 | 4 | |a nsp1 | |
650 | 4 | |a nuclear pore complex | |
650 | 7 | |a Nuclear Pore Complex Proteins |2 NLM | |
650 | 7 | |a Nup93 protein, human |2 NLM | |
650 | 7 | |a Phosphoproteins |2 NLM | |
650 | 7 | |a RNA-Binding Proteins |2 NLM | |
650 | 7 | |a Viral Nonstructural Proteins |2 NLM | |
650 | 7 | |a Nsp1 protein, SARS coronavirus |2 NLM | |
650 | 7 | |a EC 2.7.7.48 |2 NLM | |
650 | 7 | |a RNA-Dependent RNA Polymerase |2 NLM | |
650 | 7 | |a EC 2.7.7.48 |2 NLM | |
700 | 1 | |a Abrar, Fareeha |e verfasserin |4 aut | |
700 | 1 | |a Dodhia, Maya P |e verfasserin |4 aut | |
700 | 1 | |a Gonzalez, Fabiola G |e verfasserin |4 aut | |
700 | 1 | |a Nag, Anita |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Biochemistry and cell biology = Biochimie et biologie cellulaire |d 1988 |g 97(2019), 6 vom: 19. Dez., Seite 758-766 |w (DE-627)NLM012606049 |x 1208-6002 |7 nnns |
773 | 1 | 8 | |g volume:97 |g year:2019 |g number:6 |g day:19 |g month:12 |g pages:758-766 |
856 | 4 | 0 | |u http://dx.doi.org/10.1139/bcb-2018-0394 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 97 |j 2019 |e 6 |b 19 |c 12 |h 758-766 |