Highly sensitive glutathione assay and intracellular imaging with functionalized semiconductor quantum dots
Glutathione (GSH) plays a vital role in biological systems and is associated with human pathology. The engineering of semiconductor quantum dots (QDs) as fluorescent probes for GSH sensing and bioimaging is a potential yet rarely reported approach. Herein, we report the in situ growth of manganese dioxide nanosheets (MnO2) on silica-coated semiconductor quantum dots (QDSiO2), to prepare a stable and biocompatible fluorescent nanoprobe (QD@SiO2-MnO2) for the selective and sensitive detection of GSH. The modification of QD@SiO2 with MnO2 significantly quenched the fluorescence of CdSe/ZnS QDs, yet the addition of GSH efficiently recovered the fluorescence of the nanoprobe due to the decomposition of MnO2 by GSH. This nanosensor showed a rapid response to GSH with a low detection limit, and high selectivity towards GSH over potential interferences. Furthermore, the MnO2-engineered QDs had good biocompatibility and cellular uptake ability, and were successfully applied for the real-time imaging of intracellular GSH. We envision that semiconductor QD-based probes will stimulate the study of GSH dynamics and facilitate the understanding of GSH-related pathophysiological events.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Nanoscale - 11(2019), 11 vom: 14. März, Seite 5014-5020 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sun, Junlin [VerfasserIn] |
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| Links: |
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| Themen: |
7631-86-9 |
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| Anmerkungen: |
Date Completed 29.04.2019 Date Revised 29.04.2019 published: Print Citation Status MEDLINE |
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| doi: |
10.1039/c8nr09801h |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM294640398 |
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| 520 | |a Glutathione (GSH) plays a vital role in biological systems and is associated with human pathology. The engineering of semiconductor quantum dots (QDs) as fluorescent probes for GSH sensing and bioimaging is a potential yet rarely reported approach. Herein, we report the in situ growth of manganese dioxide nanosheets (MnO2) on silica-coated semiconductor quantum dots (QDSiO2), to prepare a stable and biocompatible fluorescent nanoprobe (QD@SiO2-MnO2) for the selective and sensitive detection of GSH. The modification of QD@SiO2 with MnO2 significantly quenched the fluorescence of CdSe/ZnS QDs, yet the addition of GSH efficiently recovered the fluorescence of the nanoprobe due to the decomposition of MnO2 by GSH. This nanosensor showed a rapid response to GSH with a low detection limit, and high selectivity towards GSH over potential interferences. Furthermore, the MnO2-engineered QDs had good biocompatibility and cellular uptake ability, and were successfully applied for the real-time imaging of intracellular GSH. We envision that semiconductor QD-based probes will stimulate the study of GSH dynamics and facilitate the understanding of GSH-related pathophysiological events | ||
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| 700 | 1 | |a Yu, Wenqian |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Qunying |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Jialing |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yahua |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Fuan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoqing |e verfasserin |4 aut | |
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