Transcriptome patterns in hidradenitis suppurativa : support for the role of antimicrobial peptides and interferon pathways in disease pathogenesis
© 2019 British Association of Dermatologists..
BACKGROUND: Hidradenitis suppurativa (HS) is a recurrent inflammatory disease of the apocrine sweat glands. Immune dysregulation probably contributes to the pathogenesis of HS.
AIM: To harness mRNA expression arrays to investigate the transcriptome profile in HS compared with control skin.
METHODS: Illumina® HumanHT-12 v4 Expression BeadChips were used to measure mRNA expression in skin samples from HS (n = 10) and abdominoplasty (n = 11) skin specimens. Differentially expressed genes were detected by fitting genewise linear models to the normalized expression data and then modelling using the web-based software Ingenuity® Pathway Analysis.
RESULTS: The antimicrobial peptide Dermcidin and the cytokine regulator interleukin (IL)-37 were both significantly downregulated in the HS specimens (Dermcidin expression log ratio -3.93, expression P = 0.04; IL-37 expression log ratio -3.29, expression P < 0.001). Pathway analysis revealed the interferon-signalling pathway, leucocyte extravasation pathway, T helper 1 and 2 pathways and nuclear factor of activated T cells as the top-five upregulated pathways in the HS samples.
CONCLUSION: Evaluation of transcriptome patterns in HS compared with normal skin demonstrated downregulation of the antimicrobial peptide Dermcidin and the innate immune regulator IL-37, as well as upregulation of interferon pathways and pathways of leucocyte activation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Clinical and experimental dermatology - 44(2019), 8 vom: 15. Dez., Seite 882-892 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shanmugam, V K [VerfasserIn] |
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| Links: |
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| Themen: |
9008-11-1 |
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| Anmerkungen: |
Date Completed 27.03.2020 Date Revised 27.03.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/ced.13959 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM29453122X |
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| 500 | |a Date Completed 27.03.2020 | ||
| 500 | |a Date Revised 27.03.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2019 British Association of Dermatologists. | ||
| 520 | |a BACKGROUND: Hidradenitis suppurativa (HS) is a recurrent inflammatory disease of the apocrine sweat glands. Immune dysregulation probably contributes to the pathogenesis of HS | ||
| 520 | |a AIM: To harness mRNA expression arrays to investigate the transcriptome profile in HS compared with control skin | ||
| 520 | |a METHODS: Illumina® HumanHT-12 v4 Expression BeadChips were used to measure mRNA expression in skin samples from HS (n = 10) and abdominoplasty (n = 11) skin specimens. Differentially expressed genes were detected by fitting genewise linear models to the normalized expression data and then modelling using the web-based software Ingenuity® Pathway Analysis | ||
| 520 | |a RESULTS: The antimicrobial peptide Dermcidin and the cytokine regulator interleukin (IL)-37 were both significantly downregulated in the HS specimens (Dermcidin expression log ratio -3.93, expression P = 0.04; IL-37 expression log ratio -3.29, expression P < 0.001). Pathway analysis revealed the interferon-signalling pathway, leucocyte extravasation pathway, T helper 1 and 2 pathways and nuclear factor of activated T cells as the top-five upregulated pathways in the HS samples | ||
| 520 | |a CONCLUSION: Evaluation of transcriptome patterns in HS compared with normal skin demonstrated downregulation of the antimicrobial peptide Dermcidin and the innate immune regulator IL-37, as well as upregulation of interferon pathways and pathways of leucocyte activation | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 7 | |a Interleukin-1 |2 NLM | |
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| 650 | 7 | |a Interferons |2 NLM | |
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| 700 | 1 | |a Crandall, K A |e verfasserin |4 aut | |
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