Label-free differentiation of human immunodeficiency virus-1 infected from uninfected cells using transmission measurement
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim..
Transmission measurement has been perceived as a potential candidate for label-free investigation of biological material. It is a real-time, label-free and non-invasive optical detection technique that has found wide applications in pharmaceutical industry as well as the biological and medical fields. Combining transmission measurement with optical trapping has emerged as a powerful tool allowing stable sample trapping, while also facilitating transmittance data analysis. In this study, a near-infrared laser beam emitting at a wavelength of 1064 nm was used for both optical trapping and transmission measurement investigation of human immunodeficiency virus 1 (HIV-1) infected and uninfected TZM-bl cells. The measurements of the transmittance intensity of individual cells in solution were carried out using a home built optical trapping system combined with laser transmission setup using a single beam gradient trap. Transmittance spectral intensity patterns revealed significant differences between the HIV-1 infected and uninfected cells. This result suggests that the transmittance data analysis technique used in this study has the potential to differentiate between infected and uninfected TZM-bl cells without the use of labels. The results obtained in this study could pave a way into developing an HIV-1 label-free diagnostic tool with possible applications at the point of care.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Journal of biophotonics - 12(2019), 7 vom: 21. Juli, Seite e201800349 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ombinda-Lemboumba, Saturnin [VerfasserIn] |
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| Links: |
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| Themen: |
HIV-1 infection |
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| Anmerkungen: |
Date Completed 08.09.2020 Date Revised 08.09.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/jbio.201800349 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM294364315 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | ||
| 520 | |a Transmission measurement has been perceived as a potential candidate for label-free investigation of biological material. It is a real-time, label-free and non-invasive optical detection technique that has found wide applications in pharmaceutical industry as well as the biological and medical fields. Combining transmission measurement with optical trapping has emerged as a powerful tool allowing stable sample trapping, while also facilitating transmittance data analysis. In this study, a near-infrared laser beam emitting at a wavelength of 1064 nm was used for both optical trapping and transmission measurement investigation of human immunodeficiency virus 1 (HIV-1) infected and uninfected TZM-bl cells. The measurements of the transmittance intensity of individual cells in solution were carried out using a home built optical trapping system combined with laser transmission setup using a single beam gradient trap. Transmittance spectral intensity patterns revealed significant differences between the HIV-1 infected and uninfected cells. This result suggests that the transmittance data analysis technique used in this study has the potential to differentiate between infected and uninfected TZM-bl cells without the use of labels. The results obtained in this study could pave a way into developing an HIV-1 label-free diagnostic tool with possible applications at the point of care | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a label-free detection | |
| 650 | 4 | |a mammalian cells | |
| 650 | 4 | |a optical trapping | |
| 650 | 4 | |a transmission measurement | |
| 700 | 1 | |a Malabi, Rudzani |e verfasserin |4 aut | |
| 700 | 1 | |a Lugongolo, Masixole Y |e verfasserin |4 aut | |
| 700 | 1 | |a Thobakgale, Lebogang |e verfasserin |4 aut | |
| 700 | 1 | |a Manoto, Sello L |e verfasserin |4 aut | |
| 700 | 1 | |a Mthunzi-Kufa, Patience |e verfasserin |4 aut | |
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