Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication
Assembly of paramyxoviral nucleocapsids on the RNA genome is an essential step in the viral cycle. The structural basis of this process has remained obscure due to the inability to control encapsidation. We used a recently developed approach to assemble measles virus nucleocapsid-like particles on specific sequences of RNA hexamers (poly-Adenine and viral genomic 5') in vitro, and determined their cryoelectron microscopy maps to 3.3-Å resolution. The structures unambiguously determine 5' and 3' binding sites and thereby the binding-register of viral genomic RNA within nucleocapsids. This observation reveals that the 3' end of the genome is largely exposed in fully assembled measles nucleocapsids. In particular, the final three nucleotides of the genome are rendered accessible to the RNA-dependent RNA polymerase complex, possibly enabling efficient RNA processing. The structures also reveal local and global conformational changes in the nucleoprotein upon assembly, in particular involving helix α6 and helix α13 that form edges of the RNA binding groove. Disorder is observed in the bound RNA, localized at one of the two backbone conformational switch sites. The high-resolution structure allowed us to identify putative nucleobase interaction sites in the RNA-binding groove, whose impact on assembly kinetics was measured using real-time NMR. Mutation of one of these sites, R195, whose sidechain stabilizes both backbone and base of a bound nucleic acid, is thereby shown to be essential for nucleocapsid-like particle assembly.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:116 |
|---|---|
| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 116(2019), 10 vom: 05. März, Seite 4256-4264 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Desfosses, Ambroise [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 23.03.2020 Date Revised 10.12.2020 published: Print-Electronic PDB: 6H5Q Citation Status MEDLINE |
|---|
| doi: |
10.1073/pnas.1816417116 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM294123199 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM294123199 | ||
| 003 | DE-627 | ||
| 005 | 20231225080818.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1073/pnas.1816417116 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0980.xml |
| 035 | |a (DE-627)NLM294123199 | ||
| 035 | |a (NLM)30787192 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Desfosses, Ambroise |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 23.03.2020 | ||
| 500 | |a Date Revised 10.12.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a PDB: 6H5Q | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Assembly of paramyxoviral nucleocapsids on the RNA genome is an essential step in the viral cycle. The structural basis of this process has remained obscure due to the inability to control encapsidation. We used a recently developed approach to assemble measles virus nucleocapsid-like particles on specific sequences of RNA hexamers (poly-Adenine and viral genomic 5') in vitro, and determined their cryoelectron microscopy maps to 3.3-Å resolution. The structures unambiguously determine 5' and 3' binding sites and thereby the binding-register of viral genomic RNA within nucleocapsids. This observation reveals that the 3' end of the genome is largely exposed in fully assembled measles nucleocapsids. In particular, the final three nucleotides of the genome are rendered accessible to the RNA-dependent RNA polymerase complex, possibly enabling efficient RNA processing. The structures also reveal local and global conformational changes in the nucleoprotein upon assembly, in particular involving helix α6 and helix α13 that form edges of the RNA binding groove. Disorder is observed in the bound RNA, localized at one of the two backbone conformational switch sites. The high-resolution structure allowed us to identify putative nucleobase interaction sites in the RNA-binding groove, whose impact on assembly kinetics was measured using real-time NMR. Mutation of one of these sites, R195, whose sidechain stabilizes both backbone and base of a bound nucleic acid, is thereby shown to be essential for nucleocapsid-like particle assembly | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a NMR | |
| 650 | 4 | |a assembly | |
| 650 | 4 | |a cryoelectron microscopy | |
| 650 | 4 | |a measles virus | |
| 650 | 4 | |a nucleocapsids | |
| 650 | 7 | |a Nucleocapsid Proteins |2 NLM | |
| 650 | 7 | |a Nucleoproteins |2 NLM | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 650 | 7 | |a Viral Proteins |2 NLM | |
| 650 | 7 | |a nucleoprotein, Measles virus |2 NLM | |
| 700 | 1 | |a Milles, Sigrid |e verfasserin |4 aut | |
| 700 | 1 | |a Jensen, Malene Ringkjøbing |e verfasserin |4 aut | |
| 700 | 1 | |a Guseva, Serafima |e verfasserin |4 aut | |
| 700 | 1 | |a Colletier, Jacques-Philippe |e verfasserin |4 aut | |
| 700 | 1 | |a Maurin, Damien |e verfasserin |4 aut | |
| 700 | 1 | |a Schoehn, Guy |e verfasserin |4 aut | |
| 700 | 1 | |a Gutsche, Irina |e verfasserin |4 aut | |
| 700 | 1 | |a Ruigrok, Rob W H |e verfasserin |4 aut | |
| 700 | 1 | |a Blackledge, Martin |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Proceedings of the National Academy of Sciences of the United States of America |d 1915 |g 116(2019), 10 vom: 05. März, Seite 4256-4264 |w (DE-627)NLM000008982 |x 1091-6490 |7 nnns |
| 773 | 1 | 8 | |g volume:116 |g year:2019 |g number:10 |g day:05 |g month:03 |g pages:4256-4264 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1073/pnas.1816417116 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 116 |j 2019 |e 10 |b 05 |c 03 |h 4256-4264 | ||