Structure elucidation and gene cluster characterization of the O-antigen of Vibrio cholerae O14
Copyright © 2019 Elsevier Ltd. All rights reserved..
The O-polysaccharide (O-antigen) of Vibrio cholerae O14 was studied using chemical analyses and 1D and 2D NMR spectroscopy. The following structure of the repeating unit of the O-antigen was established: where GlcpN(SHb) indicates 2-deoxy-2-[(S)-3-hydroxybutanoylamino]-d-glucose. We found that Vibrio cholerae O14 is similar to that of O-polysaccharide of Azospirillum brasilense S17, which has been reported earlier. Moreover, we predicted functions of all the genes in the O-antigen gene cluster according to the structure established. Our study enriches the existing O-antigen database of Vibrio cholerae, and further facilitates the bacterial serotype identification.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:474 |
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| Enthalten in: |
Carbohydrate research - 474(2019) vom: 15. Feb., Seite 67-71 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Perepelov, Andrei V [VerfasserIn] |
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| Links: |
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| Themen: |
Amino Sugars |
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| Anmerkungen: |
Date Completed 14.06.2019 Date Revised 14.06.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.carres.2019.01.007 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM293893519 |
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| 245 | 1 | 0 | |a Structure elucidation and gene cluster characterization of the O-antigen of Vibrio cholerae O14 |
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| 500 | |a Date Revised 14.06.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The O-polysaccharide (O-antigen) of Vibrio cholerae O14 was studied using chemical analyses and 1D and 2D NMR spectroscopy. The following structure of the repeating unit of the O-antigen was established: where GlcpN(SHb) indicates 2-deoxy-2-[(S)-3-hydroxybutanoylamino]-d-glucose. We found that Vibrio cholerae O14 is similar to that of O-polysaccharide of Azospirillum brasilense S17, which has been reported earlier. Moreover, we predicted functions of all the genes in the O-antigen gene cluster according to the structure established. Our study enriches the existing O-antigen database of Vibrio cholerae, and further facilitates the bacterial serotype identification | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bacterial polysaccharide structure | |
| 650 | 4 | |a GRASS | |
| 650 | 4 | |a Lipopolysaccharide | |
| 650 | 4 | |a O-antigen | |
| 650 | 4 | |a O-antigen gene cluster | |
| 650 | 4 | |a O-specific polysaccharide | |
| 650 | 4 | |a Vibrio cholerae | |
| 650 | 7 | |a Amino Sugars |2 NLM | |
| 650 | 7 | |a O Antigens |2 NLM | |
| 700 | 1 | |a Li, Xiaodan |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Cong |e verfasserin |4 aut | |
| 700 | 1 | |a Filatov, Andrei V |e verfasserin |4 aut | |
| 700 | 1 | |a Shashkov, Alexander S |e verfasserin |4 aut | |
| 700 | 1 | |a Senchenkova, Sofia N |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Bin |e verfasserin |4 aut | |
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