Increased Autophagy and Apoptosis in the Kidneys of Intrauterine Growth Restricted Rats
BACKGROUND: IUGR has been associated with nephron loss and chronic kidney disease (CKD).
MATERIALS AND METHODS: We examined autophagy and apoptosis markers in the kidneys of IUGR Sprague Dawley rats induced by maternal low protein diet (LP), comparing them to controls. The autophagy marker LC3B, the pro-apoptotic protein Bax, and the anti-apoptotic protein Bcl-2 were determined by quantitative immunoblotting. Immunohistochemical expressions of LC3B, Bax, and Bcl-2 were evaluated at 4 weeks age. Glomerular counts (by maceration techniques) were performed at 5 weeks.
RESULTS: The LP diet offspring were lighter (P < 0.05). In IUGR kidneys, LC3B and Bax were increased at birth (p < 0.05, p < 0.001) and at 4 weeks (p < 0.0142, p < 0.0001), Bcl-2 was decreased at birth (p < 0.05), and there were less glomeruli (p < 0.01) at 5 weeks.
CONCLUSIONS: Autophagy and apoptosis may have a role in IUGR associated decreased nephron number in Sprague rats.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:38 |
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| Enthalten in: |
Fetal and pediatric pathology - 38(2019), 3 vom: 13. Juni, Seite 185-194 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Stewart, Tyrus [VerfasserIn] |
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| Links: |
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| Themen: |
Apoptosis |
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| Anmerkungen: |
Date Completed 12.12.2019 Date Revised 17.12.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1080/15513815.2018.1564160 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM293674299 |
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| 500 | |a Date Completed 12.12.2019 | ||
| 500 | |a Date Revised 17.12.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: IUGR has been associated with nephron loss and chronic kidney disease (CKD) | ||
| 520 | |a MATERIALS AND METHODS: We examined autophagy and apoptosis markers in the kidneys of IUGR Sprague Dawley rats induced by maternal low protein diet (LP), comparing them to controls. The autophagy marker LC3B, the pro-apoptotic protein Bax, and the anti-apoptotic protein Bcl-2 were determined by quantitative immunoblotting. Immunohistochemical expressions of LC3B, Bax, and Bcl-2 were evaluated at 4 weeks age. Glomerular counts (by maceration techniques) were performed at 5 weeks | ||
| 520 | |a RESULTS: The LP diet offspring were lighter (P < 0.05). In IUGR kidneys, LC3B and Bax were increased at birth (p < 0.05, p < 0.001) and at 4 weeks (p < 0.0142, p < 0.0001), Bcl-2 was decreased at birth (p < 0.05), and there were less glomeruli (p < 0.01) at 5 weeks | ||
| 520 | |a CONCLUSIONS: Autophagy and apoptosis may have a role in IUGR associated decreased nephron number in Sprague rats | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Autophagy | |
| 650 | 4 | |a apoptosis | |
| 650 | 4 | |a intrauterine growth restriction | |
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| 700 | 1 | |a Vehaskari, V Matti |e verfasserin |4 aut | |
| 700 | 1 | |a Hodgeson, Sydney M |e verfasserin |4 aut | |
| 700 | 1 | |a Aviles, Diego H |e verfasserin |4 aut | |
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