Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion
Copyright © 2018 Elsevier Inc. All rights reserved..
Recent outbreaks of severe acute respiratory syndrome and Middle East respiratory syndrome, along with the threat of a future coronavirus-mediated pandemic, underscore the importance of finding ways to combat these viruses. The trimeric spike transmembrane glycoprotein S mediates entry into host cells and is the major target of neutralizing antibodies. To understand the humoral immune response elicited upon natural infections with coronaviruses, we structurally characterized the SARS-CoV and MERS-CoV S glycoproteins in complex with neutralizing antibodies isolated from human survivors. Although the two antibodies studied blocked attachment to the host cell receptor, only the anti-SARS-CoV S antibody triggered fusogenic conformational changes via receptor functional mimicry. These results provide a structural framework for understanding coronavirus neutralization by human antibodies and shed light on activation of coronavirus membrane fusion, which takes place through a receptor-driven ratcheting mechanism.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:176 |
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| Enthalten in: |
Cell - 176(2019), 5 vom: 21. Feb., Seite 1026-1039.e15 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Walls, Alexandra C [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 06.01.2020 Date Revised 07.12.2022 published: Print-Electronic ErratumIn: Cell. 2020 Dec 10;183(6):1732. - PMID 33306956 Citation Status MEDLINE |
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| doi: |
10.1016/j.cell.2018.12.028 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM293396604 |
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| 100 | 1 | |a Walls, Alexandra C |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion |
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| 500 | |a Date Revised 07.12.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a ErratumIn: Cell. 2020 Dec 10;183(6):1732. - PMID 33306956 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 Elsevier Inc. All rights reserved. | ||
| 520 | |a Recent outbreaks of severe acute respiratory syndrome and Middle East respiratory syndrome, along with the threat of a future coronavirus-mediated pandemic, underscore the importance of finding ways to combat these viruses. The trimeric spike transmembrane glycoprotein S mediates entry into host cells and is the major target of neutralizing antibodies. To understand the humoral immune response elicited upon natural infections with coronaviruses, we structurally characterized the SARS-CoV and MERS-CoV S glycoproteins in complex with neutralizing antibodies isolated from human survivors. Although the two antibodies studied blocked attachment to the host cell receptor, only the anti-SARS-CoV S antibody triggered fusogenic conformational changes via receptor functional mimicry. These results provide a structural framework for understanding coronavirus neutralization by human antibodies and shed light on activation of coronavirus membrane fusion, which takes place through a receptor-driven ratcheting mechanism | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a MERS-CoV | |
| 650 | 4 | |a N-linked glycosylation | |
| 650 | 4 | |a SARS-CoV | |
| 650 | 4 | |a class I fusion protein | |
| 650 | 4 | |a coronavirus | |
| 650 | 4 | |a glycoproteomics | |
| 650 | 4 | |a membrane fusion | |
| 650 | 4 | |a neutralizing antibodies | |
| 650 | 4 | |a spike glycoprotein | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Antibodies, Neutralizing |2 NLM | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Receptors, Virus |2 NLM | |
| 650 | 7 | |a Spike Glycoprotein, Coronavirus |2 NLM | |
| 700 | 1 | |a Xiong, Xiaoli |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Young-Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Tortorici, M Alejandra |e verfasserin |4 aut | |
| 700 | 1 | |a Snijder, Joost |e verfasserin |4 aut | |
| 700 | 1 | |a Quispe, Joel |e verfasserin |4 aut | |
| 700 | 1 | |a Cameroni, Elisabetta |e verfasserin |4 aut | |
| 700 | 1 | |a Gopal, Robin |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Mian |e verfasserin |4 aut | |
| 700 | 1 | |a Lanzavecchia, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Zambon, Maria |e verfasserin |4 aut | |
| 700 | 1 | |a Rey, Félix A |e verfasserin |4 aut | |
| 700 | 1 | |a Corti, Davide |e verfasserin |4 aut | |
| 700 | 1 | |a Veesler, David |e verfasserin |4 aut | |
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