Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay
Copyright © 2019 Elsevier B.V. All rights reserved..
Polyhalogenated carbazoles (PHCZs), which have the similar structure of dioxin, have been reported ubiquitous in the environments and drawn wide concerns. However, their potential ecological and health risks are still poorly understood. Here, wildtype zebrafish embryos were used to evaluate the environmental risks of 2,7-dibromocarbazole (2,7-DBCZ), 3,6-dibromocarbazole (3,6-DBCZ), and 3,6-dichlorocarbazole (3,6-DCCZ). 2,7-DBCZ was the most toxic compound with the 96-h LC50 value of 581.8 ± 29.3 μg·L-1 and the EC50 value of 201.5 ± 6.5 μg·L-1 for pericardial edema. The teratogenic effects of 2,7-DBCZ were tested using transgenic zebrafish larvae. The transcriptomic analysis revealed that 90 genes in zebrafish expressed differently after exposure to 2,7-DBCZ, and many pathways were related to aryl hydrocarbon receptor (AhR) activation. The qRT-PCR also showed that expression levels of AhR1 and CYP1 A in zebrafish were significantly up-regulated after exposure to 2,7-DBCZ. In conclusion, 2,7-DBCZ exhibited more potent toxicity and cardiac teratogenic effects, and presented developmental toxicity partially consistent with AhR activation. Our results will be of great help to the risk assessment and regulation-making of PHCZs. Meanwhile, further studies should be promoted to illustrate the potential mechanism between PHCZs and AhR in the near future.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:368 |
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| Enthalten in: |
Journal of hazardous materials - 368(2019) vom: 15. Apr., Seite 514-522 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ji, Chenyang [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 30.09.2020 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jhazmat.2019.01.079 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM293375895 |
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| 520 | |a Copyright © 2019 Elsevier B.V. All rights reserved. | ||
| 520 | |a Polyhalogenated carbazoles (PHCZs), which have the similar structure of dioxin, have been reported ubiquitous in the environments and drawn wide concerns. However, their potential ecological and health risks are still poorly understood. Here, wildtype zebrafish embryos were used to evaluate the environmental risks of 2,7-dibromocarbazole (2,7-DBCZ), 3,6-dibromocarbazole (3,6-DBCZ), and 3,6-dichlorocarbazole (3,6-DCCZ). 2,7-DBCZ was the most toxic compound with the 96-h LC50 value of 581.8 ± 29.3 μg·L-1 and the EC50 value of 201.5 ± 6.5 μg·L-1 for pericardial edema. The teratogenic effects of 2,7-DBCZ were tested using transgenic zebrafish larvae. The transcriptomic analysis revealed that 90 genes in zebrafish expressed differently after exposure to 2,7-DBCZ, and many pathways were related to aryl hydrocarbon receptor (AhR) activation. The qRT-PCR also showed that expression levels of AhR1 and CYP1 A in zebrafish were significantly up-regulated after exposure to 2,7-DBCZ. In conclusion, 2,7-DBCZ exhibited more potent toxicity and cardiac teratogenic effects, and presented developmental toxicity partially consistent with AhR activation. Our results will be of great help to the risk assessment and regulation-making of PHCZs. Meanwhile, further studies should be promoted to illustrate the potential mechanism between PHCZs and AhR in the near future | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a 2,7-Dibromocarbazole | |
| 650 | 4 | |a Cardiac teratogenic effect | |
| 650 | 4 | |a Polyhalogenated carbazoles | |
| 650 | 4 | |a Transcriptomics | |
| 650 | 4 | |a Transgenic zebrafish | |
| 650 | 7 | |a Carbazoles |2 NLM | |
| 650 | 7 | |a Teratogens |2 NLM | |
| 650 | 7 | |a Zebrafish Proteins |2 NLM | |
| 700 | 1 | |a Yan, Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yuanchen |e verfasserin |4 aut | |
| 700 | 1 | |a Yue, Siqing |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Qiaoxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jiangfei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Meirong |e verfasserin |4 aut | |
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