Dual-phone illumination-imaging system for high resolution and large field of view multi-modal microscopy
In this paper we present for the first time a system comprised of two mobile phones, one for illumination and the other for microscopy, as a portable, user-friendly, and cost-effective microscopy platform for a wide range of applications. Versatile and adaptive illumination is made with a Retina display of an Apple mobile phone device. The phone screen is used to project various illumination patterns onto the specimen being imaged, each corresponding to a different illumination mode, such as bright-field, dark-field, point illumination, Rheinberg illumination, and fluorescence microscopy. The second phone (a Nokia phone) is modified to record microscopic images about the sample. This imaging platform provides a high spatial resolution of at least 2 μm, a large field-of-view of 3.6 × 2.7 mm, and a working distance of 0.6 mm. We demonstrate the performance of this platform for the visualization of microorganisms within microfluidic devices to gather qualitative and quantitative information regarding microorganism morphology, dimension, count, and velocity/trajectories in the x-y plane.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
|---|---|
| Enthalten in: |
Lab on a chip - 19(2019), 5 vom: 26. Feb., Seite 825-836 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kheireddine, Sara [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Journal Article |
|---|
| Anmerkungen: |
Date Revised 20.11.2019 published: Print Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1039/c8lc00995c |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM293251649 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM293251649 | ||
| 003 | DE-627 | ||
| 005 | 20231225074919.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1039/c8lc00995c |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0977.xml |
| 035 | |a (DE-627)NLM293251649 | ||
| 035 | |a (NLM)30698180 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kheireddine, Sara |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Dual-phone illumination-imaging system for high resolution and large field of view multi-modal microscopy |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 20.11.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a In this paper we present for the first time a system comprised of two mobile phones, one for illumination and the other for microscopy, as a portable, user-friendly, and cost-effective microscopy platform for a wide range of applications. Versatile and adaptive illumination is made with a Retina display of an Apple mobile phone device. The phone screen is used to project various illumination patterns onto the specimen being imaged, each corresponding to a different illumination mode, such as bright-field, dark-field, point illumination, Rheinberg illumination, and fluorescence microscopy. The second phone (a Nokia phone) is modified to record microscopic images about the sample. This imaging platform provides a high spatial resolution of at least 2 μm, a large field-of-view of 3.6 × 2.7 mm, and a working distance of 0.6 mm. We demonstrate the performance of this platform for the visualization of microorganisms within microfluidic devices to gather qualitative and quantitative information regarding microorganism morphology, dimension, count, and velocity/trajectories in the x-y plane | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 700 | 1 | |a Sudalaiyadum Perumal, Ayyappasamy |e verfasserin |4 aut | |
| 700 | 1 | |a Smith, Zachary J |e verfasserin |4 aut | |
| 700 | 1 | |a Nicolau, Dan V |e verfasserin |4 aut | |
| 700 | 1 | |a Wachsmann-Hogiu, Sebastian |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Lab on a chip |d 2001 |g 19(2019), 5 vom: 26. Feb., Seite 825-836 |w (DE-627)NLM147083753 |x 1473-0189 |7 nnns |
| 773 | 1 | 8 | |g volume:19 |g year:2019 |g number:5 |g day:26 |g month:02 |g pages:825-836 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1039/c8lc00995c |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 19 |j 2019 |e 5 |b 26 |c 02 |h 825-836 | ||