CONSTRUCTION OF A TEST-SYSTEM WITH NANOPARTICLES OF COLLOID SILVER FOR DETECTION OF PSEUDOTUBERCULOSIS AND INTESTINAL YERSINIOSIS FOR CAUSATIVE AGENTS IN DOT-IMMUNOASSAY
AIM: Construction of an immunologic test-system for detection of causative agents of enteropathogenic Yersinia (Yersinia pseudotuberculosis and Y. enterocolitica) by dot-immunoassay.
MATERIALS AND METHODS: Nenoparticles of colloid silver sized 5-9 nm were used as a marker of specific antibodies. IgG fraction was isolated from commercial antisera to Y. pseudotuberculosis (Ο:1) and Y. enterocolitica (Ο:3 and Ο:9). Testing of the obtained test-system was carried out on 20 strains of Y.pseudotuberculosis and Y. enterocolitica (10 of each species).
RESULTS: Dot-analysis had a specific character and detected enteropathogenic Yersinia at a level of 5-10⁵ - 8.10⁶ CFU/ml (100 - 1000 CFU in sample). Wherein cross-reaction with heterologic studied microorganisms - Escherichia coli, Salmonella typhimurium, Shigella flexneri, Yersina pestis EV - as not observed. A possibility of simultaneous detection and serotyping of Y. enterocolitica is shown, that is necessary for confirmation of their epidemic significance.
CONCLUSION: The developed test-systems allow to study micro volumes of the samples under study (1 μ1), are express (1.5 - 2h), highly sensitive and specific, technically simple and do not require the use of high-cost equipment, special training of the staff, may be successfully used in practical healthcar in laboratories with varying equiptment levels.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2017 |
|---|---|
| Enthalten in: |
Zhurnal mikrobiologii, epidemiologii i immunobiologii - (2017), 1 vom: 18. Jan., Seite 55-61 |
| Sprache: |
Russisch |
|---|
| Beteiligte Personen: |
Zagoskina, T Yu [VerfasserIn] |
|---|
| Themen: |
3M4G523W1G |
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| Anmerkungen: |
Date Completed 19.02.2019 Date Revised 16.07.2020 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM293225311 |
|---|
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| 100 | 1 | |a Zagoskina, T Yu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a CONSTRUCTION OF A TEST-SYSTEM WITH NANOPARTICLES OF COLLOID SILVER FOR DETECTION OF PSEUDOTUBERCULOSIS AND INTESTINAL YERSINIOSIS FOR CAUSATIVE AGENTS IN DOT-IMMUNOASSAY |
| 264 | 1 | |c 2017 | |
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| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 19.02.2019 | ||
| 500 | |a Date Revised 16.07.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a AIM: Construction of an immunologic test-system for detection of causative agents of enteropathogenic Yersinia (Yersinia pseudotuberculosis and Y. enterocolitica) by dot-immunoassay | ||
| 520 | |a MATERIALS AND METHODS: Nenoparticles of colloid silver sized 5-9 nm were used as a marker of specific antibodies. IgG fraction was isolated from commercial antisera to Y. pseudotuberculosis (Ο:1) and Y. enterocolitica (Ο:3 and Ο:9). Testing of the obtained test-system was carried out on 20 strains of Y.pseudotuberculosis and Y. enterocolitica (10 of each species) | ||
| 520 | |a RESULTS: Dot-analysis had a specific character and detected enteropathogenic Yersinia at a level of 5-10⁵ - 8.10⁶ CFU/ml (100 - 1000 CFU in sample). Wherein cross-reaction with heterologic studied microorganisms - Escherichia coli, Salmonella typhimurium, Shigella flexneri, Yersina pestis EV - as not observed. A possibility of simultaneous detection and serotyping of Y. enterocolitica is shown, that is necessary for confirmation of their epidemic significance | ||
| 520 | |a CONCLUSION: The developed test-systems allow to study micro volumes of the samples under study (1 μ1), are express (1.5 - 2h), highly sensitive and specific, technically simple and do not require the use of high-cost equipment, special training of the staff, may be successfully used in practical healthcar in laboratories with varying equiptment levels | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antibodies, Bacterial |2 NLM | |
| 650 | 7 | |a Colloids |2 NLM | |
| 650 | 7 | |a Immunoglobulin G |2 NLM | |
| 650 | 7 | |a Silver |2 NLM | |
| 650 | 7 | |a 3M4G523W1G |2 NLM | |
| 700 | 1 | |a Chesnokova, M V |e verfasserin |4 aut | |
| 700 | 1 | |a Klimov, V T |e verfasserin |4 aut | |
| 700 | 1 | |a Popova, Yu O |e verfasserin |4 aut | |
| 700 | 1 | |a Markov, E Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Starikova, O A |e verfasserin |4 aut | |
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