DETERMINATION OF PHYLOGENETIC RELATIONSHIP OF YERSINIA PESTIS STRAINS FROM NATURAL PLAGUE FOCI OF THE CAUCASUS BY MULTI-LO- CUS VNTR-ANALYSIS
AIM: Determination of the degree of phylogenetic relationship of Yersinia pestis strains iso- lated from the territories of natural foci of plague from the Caucasus using VNTR-typing by 25 loci (MLVA25).
MATERIALS AND METHODS: 26 strains of Y pestis from Russian natural foci of the Caucasus were used in the study. 25 loci of tandem repeats in Y pestis genome by Le Fleche scheme were used for execution of multi-locus VNTR-analysis. Deciphering of nucleotide sequences was carried out in automatic sequencer ABI 3130 Genetic Analyser. Analysis of confinement of clusters to certain territories, objects and time of isolation of strains was carried out. using Arc GIS 10.1 program.
RESULTS: Groups of MLVA25-types of various levels of discrimination were formed: clusters, groups and subgroups. Clusters were formed by strains ofvarious taxonomic membership: main and subspecies of Y pestis. Subgroups reflect membership of strains in certain foci, and MLVA25-types - the degree of genetic relationship.
CONCLUSION: Genetic <<portraits>> of plague causative agents obtained using MLVA25-types circulating in various natural-focal territories allow to solve problems ofboth theoretical and practical character: from interpretation of microevolution processes to the search of the source of infection and ways of its spread during possible epidemic complications.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2017 |
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Erschienen: |
2017 |
Enthalten in: |
Zur Gesamtaufnahme - year:2017 |
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Enthalten in: |
Zhurnal mikrobiologii, epidemiologii i immunobiologii - (2017), 1 vom: 18. Jan., Seite 35-41 |
Sprache: |
Russisch |
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Beteiligte Personen: |
Serdyuk, N S [VerfasserIn] |
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Themen: |
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Anmerkungen: |
Date Completed 19.02.2019 Date Revised 16.07.2020 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM29322529X |
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100 | 1 | |a Serdyuk, N S |e verfasserin |4 aut | |
245 | 1 | 0 | |a DETERMINATION OF PHYLOGENETIC RELATIONSHIP OF YERSINIA PESTIS STRAINS FROM NATURAL PLAGUE FOCI OF THE CAUCASUS BY MULTI-LO- CUS VNTR-ANALYSIS |
264 | 1 | |c 2017 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 19.02.2019 | ||
500 | |a Date Revised 16.07.2020 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a AIM: Determination of the degree of phylogenetic relationship of Yersinia pestis strains iso- lated from the territories of natural foci of plague from the Caucasus using VNTR-typing by 25 loci (MLVA25) | ||
520 | |a MATERIALS AND METHODS: 26 strains of Y pestis from Russian natural foci of the Caucasus were used in the study. 25 loci of tandem repeats in Y pestis genome by Le Fleche scheme were used for execution of multi-locus VNTR-analysis. Deciphering of nucleotide sequences was carried out in automatic sequencer ABI 3130 Genetic Analyser. Analysis of confinement of clusters to certain territories, objects and time of isolation of strains was carried out. using Arc GIS 10.1 program | ||
520 | |a RESULTS: Groups of MLVA25-types of various levels of discrimination were formed: clusters, groups and subgroups. Clusters were formed by strains ofvarious taxonomic membership: main and subspecies of Y pestis. Subgroups reflect membership of strains in certain foci, and MLVA25-types - the degree of genetic relationship | ||
520 | |a CONCLUSION: Genetic <<portraits>> of plague causative agents obtained using MLVA25-types circulating in various natural-focal territories allow to solve problems ofboth theoretical and practical character: from interpretation of microevolution processes to the search of the source of infection and ways of its spread during possible epidemic complications | ||
650 | 4 | |a Journal Article | |
700 | 1 | |a Evchenko, Yu M |e verfasserin |4 aut | |
700 | 1 | |a Kuznetsova, I V |e verfasserin |4 aut | |
700 | 1 | |a Zhilchenko, E B |e verfasserin |4 aut | |
700 | 1 | |a Zharinova, N V |e verfasserin |4 aut | |
700 | 1 | |a Konyaeva, O A |e verfasserin |4 aut | |
700 | 1 | |a Mezentsev, V M |e verfasserin |4 aut | |
700 | 1 | |a Volynkina, A S |e verfasserin |4 aut | |
700 | 1 | |a Kotenev, E S |e verfasserin |4 aut | |
700 | 1 | |a Platonov, M E |e verfasserin |4 aut | |
700 | 1 | |a Anisimov, A P |e verfasserin |4 aut | |
700 | 1 | |a Kulichenko, A N |e verfasserin |4 aut | |
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