Core-Shell Modeling of Light Scattering by Vesicles : Effect of Size, Contents, and Lamellarity
Copyright © 2019 Biophysical Society. Published by Elsevier Inc. All rights reserved..
Having a fast, reliable method for characterizing vesicles is vital for their use as model cell membranes in biophysics, synthetic biology, and origins of life studies. Instead of the traditionally used Rayleigh-Gans-Debye approximation, we use an exact extended Lorenz-Mie solution for how core-shell particles scatter light to model vesicle turbidity. This approach enables accurate interpretations of simple turbidimetric measurements and is able to accurately model highly scattering vesicles, such as larger vesicles, those with multiple layers, and those with encapsulated material. We uncover several surprising features, including that vesicle lamellarity has a larger effect on sample turbidity than vesicle size and that the technique can be used to measure the membrane thickness of vesicles. We also examine potential misinterpretations of turbidimetry and discuss when measurements are limited by forward and multiple scattering and by the geometry of the instrument.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:116 |
|---|---|
| Enthalten in: |
Biophysical journal - 116(2019), 4 vom: 19. Feb., Seite 659-669 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Anna [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
1-palmitoyl-2-oleoylphosphatidylcholine |
|---|
| Anmerkungen: |
Date Completed 21.01.2020 Date Revised 06.10.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.bpj.2019.01.006 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM293138109 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM293138109 | ||
| 003 | DE-627 | ||
| 005 | 20231225074648.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bpj.2019.01.006 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0977.xml |
| 035 | |a (DE-627)NLM293138109 | ||
| 035 | |a (NLM)30686489 | ||
| 035 | |a (PII)S0006-3495(19)30022-0 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Anna |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Core-Shell Modeling of Light Scattering by Vesicles |b Effect of Size, Contents, and Lamellarity |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 21.01.2020 | ||
| 500 | |a Date Revised 06.10.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019 Biophysical Society. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Having a fast, reliable method for characterizing vesicles is vital for their use as model cell membranes in biophysics, synthetic biology, and origins of life studies. Instead of the traditionally used Rayleigh-Gans-Debye approximation, we use an exact extended Lorenz-Mie solution for how core-shell particles scatter light to model vesicle turbidity. This approach enables accurate interpretations of simple turbidimetric measurements and is able to accurately model highly scattering vesicles, such as larger vesicles, those with multiple layers, and those with encapsulated material. We uncover several surprising features, including that vesicle lamellarity has a larger effect on sample turbidity than vesicle size and that the technique can be used to measure the membrane thickness of vesicles. We also examine potential misinterpretations of turbidimetry and discuss when measurements are limited by forward and multiple scattering and by the geometry of the instrument | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 7 | |a Lipid Bilayers |2 NLM | |
| 650 | 7 | |a Phosphatidylcholines |2 NLM | |
| 650 | 7 | |a Oleic Acid |2 NLM | |
| 650 | 7 | |a 2UMI9U37CP |2 NLM | |
| 650 | 7 | |a 1-palmitoyl-2-oleoylphosphatidylcholine |2 NLM | |
| 650 | 7 | |a TE895536Y5 |2 NLM | |
| 700 | 1 | |a Chan Miller, Christopher |e verfasserin |4 aut | |
| 700 | 1 | |a Szostak, Jack W |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biophysical journal |d 1960 |g 116(2019), 4 vom: 19. Feb., Seite 659-669 |w (DE-627)NLM000067571 |x 1542-0086 |7 nnns |
| 773 | 1 | 8 | |g volume:116 |g year:2019 |g number:4 |g day:19 |g month:02 |g pages:659-669 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bpj.2019.01.006 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 116 |j 2019 |e 4 |b 19 |c 02 |h 659-669 | ||