Inhibition of neutrophil migration and reduction of oxidative stress by ethyl p-coumarate in acute and chronic inflammatory models
Copyright © 2018. Published by Elsevier GmbH..
BACKGROUND: It is well known that medicinal plants and their products are relevant candidates for the treatment of inflammatory conditions. Ethyl p-coumarate is a phenylpropanoid that has similar structure to others anti-inflammatory and antioxidant substances. However, these activities have never been tested.
PURPOSE: The aim of this study was to investigate the effect of ethyl p-coumarate on inflammatory and oxidative stress parameters.
STUDY DESIGN: This is an experimental study to evaluate the anti-inflammatory and antioxidant activities of ethyl p-coumarate in acute and chronic models of inflammation.
METHODS: The anti-inflammatory effect of ethyl p-coumarate was evaluated in Swiss mice by carrageenan-induced paw edema model (1%, 50 μl), followed by histological analysis, and edema induced by compound 48/80 (12 µg/paw), histamine (100 µg/paw), serotonin (100 µg/paw) and prostaglandin E2 (3 nmol/paw) in comparison to indomethacin treatment (10 mg/kg, p.o.). In addition, peritonitis was induced by carrageenan (500 μg/cavity) to neutrophil and total leukocytes counting, myeloperoxidase (MPO), interleukin 6 (IL-6) and 8 (IL-8), nitrite (NO2-), glutathione (GSH) and malondialdehyde (MDA) measurements. The arthritis model was induced with Freund's complete adjuvant (id. 0.1 ml) in female Wistar rats, with measurement of joint diameter and X-ray. Changes in gastric tissue of Swiss mice were analyzed in comparison to indomethacin (20 mg/kg, p.o.).
RESULTS: After treatment with ethyl p-coumarate, the animals had no apparent toxic effects, and significantly inhibited paw edema induced by edematogenic agents, neutrophil (p < 0.001) and total leukocyte (p < 0.001) migration, MPO (p < 0.01), IL-6 (p < 0.05) and IL-8 (p < 0.5), MDA (p < 0.5), GSH (p < 0.5), NO2- (p < 0.001), joint thickness and bones changes. Furthermore, were not observed significant formation of gastric lesions.
CONCLUSION: Taken together, these results suggest that ethyl p-coumarate exhibits anti-inflammatory activity through the inhibition of inflammatory mediators and leukocyte migration without causing gastric lesions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:57 |
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| Enthalten in: |
Phytomedicine : international journal of phytotherapy and phytopharmacology - 57(2019) vom: 01. Apr., Seite 9-17 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Filho, Antônio Carlos Melo Lima [VerfasserIn] |
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| Links: |
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| Themen: |
9000-07-1 |
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| Anmerkungen: |
Date Completed 15.07.2019 Date Revised 15.07.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.phymed.2018.09.034 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM292959419 |
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| 100 | 1 | |a Filho, Antônio Carlos Melo Lima |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Inhibition of neutrophil migration and reduction of oxidative stress by ethyl p-coumarate in acute and chronic inflammatory models |
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| 500 | |a Date Completed 15.07.2019 | ||
| 500 | |a Date Revised 15.07.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018. Published by Elsevier GmbH. | ||
| 520 | |a BACKGROUND: It is well known that medicinal plants and their products are relevant candidates for the treatment of inflammatory conditions. Ethyl p-coumarate is a phenylpropanoid that has similar structure to others anti-inflammatory and antioxidant substances. However, these activities have never been tested | ||
| 520 | |a PURPOSE: The aim of this study was to investigate the effect of ethyl p-coumarate on inflammatory and oxidative stress parameters | ||
| 520 | |a STUDY DESIGN: This is an experimental study to evaluate the anti-inflammatory and antioxidant activities of ethyl p-coumarate in acute and chronic models of inflammation | ||
| 520 | |a METHODS: The anti-inflammatory effect of ethyl p-coumarate was evaluated in Swiss mice by carrageenan-induced paw edema model (1%, 50 μl), followed by histological analysis, and edema induced by compound 48/80 (12 µg/paw), histamine (100 µg/paw), serotonin (100 µg/paw) and prostaglandin E2 (3 nmol/paw) in comparison to indomethacin treatment (10 mg/kg, p.o.). In addition, peritonitis was induced by carrageenan (500 μg/cavity) to neutrophil and total leukocytes counting, myeloperoxidase (MPO), interleukin 6 (IL-6) and 8 (IL-8), nitrite (NO2-), glutathione (GSH) and malondialdehyde (MDA) measurements. The arthritis model was induced with Freund's complete adjuvant (id. 0.1 ml) in female Wistar rats, with measurement of joint diameter and X-ray. Changes in gastric tissue of Swiss mice were analyzed in comparison to indomethacin (20 mg/kg, p.o.) | ||
| 520 | |a RESULTS: After treatment with ethyl p-coumarate, the animals had no apparent toxic effects, and significantly inhibited paw edema induced by edematogenic agents, neutrophil (p < 0.001) and total leukocyte (p < 0.001) migration, MPO (p < 0.01), IL-6 (p < 0.05) and IL-8 (p < 0.5), MDA (p < 0.5), GSH (p < 0.5), NO2- (p < 0.001), joint thickness and bones changes. Furthermore, were not observed significant formation of gastric lesions | ||
| 520 | |a CONCLUSION: Taken together, these results suggest that ethyl p-coumarate exhibits anti-inflammatory activity through the inhibition of inflammatory mediators and leukocyte migration without causing gastric lesions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Arthritis | |
| 650 | 4 | |a Cytokines | |
| 650 | 4 | |a Inflammation | |
| 650 | 4 | |a Neutrophils | |
| 650 | 4 | |a Phenylpropanoid | |
| 650 | 7 | |a Anti-Inflammatory Agents, Non-Steroidal |2 NLM | |
| 650 | 7 | |a Antioxidants |2 NLM | |
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| 650 | 7 | |a Carrageenan |2 NLM | |
| 650 | 7 | |a 9000-07-1 |2 NLM | |
| 650 | 7 | |a Freund's Adjuvant |2 NLM | |
| 650 | 7 | |a 9007-81-2 |2 NLM | |
| 700 | 1 | |a Silva, Irismara Sousa |e verfasserin |4 aut | |
| 700 | 1 | |a Sousa, Francisca Beatriz Melo |e verfasserin |4 aut | |
| 700 | 1 | |a de Souza, Luan Kelves Miranda |e verfasserin |4 aut | |
| 700 | 1 | |a Gomes, Bruno da Silva |e verfasserin |4 aut | |
| 700 | 1 | |a Gonçalves, Rodrigo Lopes Gomes |e verfasserin |4 aut | |
| 700 | 1 | |a de Rezende, Diana Carvalho |e verfasserin |4 aut | |
| 700 | 1 | |a Cunha, Francisco Valmor Macedo |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Deysi Viviana Tenazoa |e verfasserin |4 aut | |
| 700 | 1 | |a Júnior, Roberto César Pereira Lima |e verfasserin |4 aut | |
| 700 | 1 | |a Medeiros, Jand Venes-Rolim |e verfasserin |4 aut | |
| 700 | 1 | |a de Sousa, Damião Pergentino |e verfasserin |4 aut | |
| 700 | 1 | |a Oliveira, Francisco de Assis |e verfasserin |4 aut | |
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