Rohitukine inhibits NF-κB activation induced by LPS and other inflammatory agents
Copyright © 2019 Elsevier B.V. All rights reserved..
Rohitukine (referred to as RHK) is a bioactive chromone alkaloid isolated from the leaves of plant Dysoxylum binectariferum, which has been reported to possess diverse pharmacological properties for the treatment of inflammatory bowel disease (IBD), diarrhoea and anti-lipidemic. However, the underlying mechanism by which RHK exerts its anti-inflammatory activity has not yet demonstrated. This study aimed to elucidate the anti-inflammatory mechanism of RHK using lipopolysaccharide (LPS) - stimulated J774A.1 macrophage cells and in-vivo inflammatory models. Results demonstrated that RHK treatment could significantly decrease the LPS-induced production of nitric oxide, prostaglandin E2 (PGE2), interleukins (ILs) and tumour necrosis factor (TNF)-α in J774A.1 cells. Molecular studies revealed that RHK inhibited the activation of upstream mediator nuclear factor-κB by suppressing the phosphorylation of IκBα and p65. In in-vivo experiments showed prominent anti-inflammatory activity of RHK. Thus, RHK could be considered as a promising candidate for the treatment of inflammatory diseases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:69 |
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| Enthalten in: |
International immunopharmacology - 69(2019) vom: 15. Apr., Seite 34-49 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Singh, Amarinder [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.07.2019 Date Revised 18.07.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.intimp.2019.01.015 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM292927339 |
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| 245 | 1 | 0 | |a Rohitukine inhibits NF-κB activation induced by LPS and other inflammatory agents |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019 Elsevier B.V. All rights reserved. | ||
| 520 | |a Rohitukine (referred to as RHK) is a bioactive chromone alkaloid isolated from the leaves of plant Dysoxylum binectariferum, which has been reported to possess diverse pharmacological properties for the treatment of inflammatory bowel disease (IBD), diarrhoea and anti-lipidemic. However, the underlying mechanism by which RHK exerts its anti-inflammatory activity has not yet demonstrated. This study aimed to elucidate the anti-inflammatory mechanism of RHK using lipopolysaccharide (LPS) - stimulated J774A.1 macrophage cells and in-vivo inflammatory models. Results demonstrated that RHK treatment could significantly decrease the LPS-induced production of nitric oxide, prostaglandin E2 (PGE2), interleukins (ILs) and tumour necrosis factor (TNF)-α in J774A.1 cells. Molecular studies revealed that RHK inhibited the activation of upstream mediator nuclear factor-κB by suppressing the phosphorylation of IκBα and p65. In in-vivo experiments showed prominent anti-inflammatory activity of RHK. Thus, RHK could be considered as a promising candidate for the treatment of inflammatory diseases | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Dysoxylum binectariferum | |
| 650 | 4 | |a Inflammation | |
| 650 | 4 | |a Macrophage | |
| 650 | 4 | |a NF-κB | |
| 650 | 4 | |a Rohitukine | |
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| 700 | 1 | |a Chibber, Pankaj |e verfasserin |4 aut | |
| 700 | 1 | |a Kolimi, Praveen |e verfasserin |4 aut | |
| 700 | 1 | |a Malik, Tanveer Ahmad |e verfasserin |4 aut | |
| 700 | 1 | |a Kapoor, Nitika |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Amit |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Narinder |e verfasserin |4 aut | |
| 700 | 1 | |a Gandhi, Sumit G |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Surjeet |e verfasserin |4 aut | |
| 700 | 1 | |a Abdullah, Sheikh Tasduq |e verfasserin |4 aut | |
| 700 | 1 | |a Vishwakarma, Ram |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Gurdarshan |e verfasserin |4 aut | |
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