Volumetric two-photon microscopy with a non-diffracting Airy beam
We demonstrate a volumetric two-photon microscopy (TPM) using the non-diffracting Airy beam as illumination. Direct mapping of the imaging trajectory shows that the Airy beam extends the axial imaging range around six times longer than a traditional Gaussian beam does along the propagation direction, while maintaining a comparable lateral width. Benefiting from its non-diffracting nature, the TPM with Airy beam illumination is able not only to capture a volumetric image within a single frame, but also to acquire image structures behind a strongly scattered medium. The volumetric specimen is mapped layer by layer under Gaussian mode, while the three-dimensional structure is projected to a single two-dimensional image under Airy mode, leading to a significantly increased acquisition speed. The performance of the TPM is evaluated employing a phantom of agarose gel imbedding fluorescent beads as well as a mouse brain slice. Finally, we showcase the penetration ability of the developed Airy TPM by imaging through a scattering environment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Optics letters - 44(2019), 2 vom: 15. Jan., Seite 391-394 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tan, Xiao-Jie [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.02.2019 Date Revised 15.02.2019 published: Print Citation Status MEDLINE |
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| doi: |
10.1364/OL.44.000391 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM292729367 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We demonstrate a volumetric two-photon microscopy (TPM) using the non-diffracting Airy beam as illumination. Direct mapping of the imaging trajectory shows that the Airy beam extends the axial imaging range around six times longer than a traditional Gaussian beam does along the propagation direction, while maintaining a comparable lateral width. Benefiting from its non-diffracting nature, the TPM with Airy beam illumination is able not only to capture a volumetric image within a single frame, but also to acquire image structures behind a strongly scattered medium. The volumetric specimen is mapped layer by layer under Gaussian mode, while the three-dimensional structure is projected to a single two-dimensional image under Airy mode, leading to a significantly increased acquisition speed. The performance of the TPM is evaluated employing a phantom of agarose gel imbedding fluorescent beads as well as a mouse brain slice. Finally, we showcase the penetration ability of the developed Airy TPM by imaging through a scattering environment | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Kong, Cihang |e verfasserin |4 aut | |
| 700 | 1 | |a Ren, Yu-Xuan |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Cora S W |e verfasserin |4 aut | |
| 700 | 1 | |a Tsia, Kevin K |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Kenneth K Y |e verfasserin |4 aut | |
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