Response surface optimisation of antioxidant extraction from kinema, a Bacillus-fermented soybean food
Copyright © 2011 Elsevier Ltd. All rights reserved..
The objective of this work was to optimise different parameters (solvent concentration, time and temperature) for antioxidant extraction from kinema, demonstrated by total phenol content (TPC) and DPPH-scavenging activity (DSA), using response surface methodology. A central composite design was performed to determine the effect of solvent concentration (methanol, 30-100%), temperature (30-60°C) and time (30-150min) on the TPC and DSA of the extract. The solvent concentration and temperature had the most significant (p<0.05) effect. The optimum conditions for the TPC extraction and DSA were 100% methanol, 50°C and 30min, in which 140mg of gallic acid equivalent (GAE) g-1 lyophilised extract and 52% DSA were predicted, while 135mg GAE g-1 lyophilised extract and 56% DSA were experimentally obtained. No significant difference (p<0.05) was found between the experimental and predicted values of the response variables.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2011 |
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| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:129 |
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| Enthalten in: |
Food chemistry - 129(2011), 2 vom: 15. Nov., Seite 507-513 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Saha, Jayati [VerfasserIn] |
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| Links: |
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| Themen: |
Antioxidant extraction |
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| Anmerkungen: |
Date Revised 20.11.2019 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.foodchem.2011.04.108 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM292624565 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Copyright © 2011 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The objective of this work was to optimise different parameters (solvent concentration, time and temperature) for antioxidant extraction from kinema, demonstrated by total phenol content (TPC) and DPPH-scavenging activity (DSA), using response surface methodology. A central composite design was performed to determine the effect of solvent concentration (methanol, 30-100%), temperature (30-60°C) and time (30-150min) on the TPC and DSA of the extract. The solvent concentration and temperature had the most significant (p<0.05) effect. The optimum conditions for the TPC extraction and DSA were 100% methanol, 50°C and 30min, in which 140mg of gallic acid equivalent (GAE) g-1 lyophilised extract and 52% DSA were predicted, while 135mg GAE g-1 lyophilised extract and 56% DSA were experimentally obtained. No significant difference (p<0.05) was found between the experimental and predicted values of the response variables | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antioxidant extraction | |
| 650 | 4 | |a Central composite design | |
| 650 | 4 | |a DPPH()-scavenging activity | |
| 650 | 4 | |a Kinema | |
| 650 | 4 | |a Total phenol content | |
| 700 | 1 | |a Biswas, Anup |e verfasserin |4 aut | |
| 700 | 1 | |a Chhetri, Abhijit |e verfasserin |4 aut | |
| 700 | 1 | |a Sarkar, Prabir K |e verfasserin |4 aut | |
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