Label-free identification of trace microcystin-LR with surface-enhanced Raman scattering spectra
Copyright © 2018 Elsevier B.V. All rights reserved..
The analysis of trace microcystin-LR (MC-LR) plays important roles in environmental fields, especially in monitoring domestic water quality and safety, since it has particularly harmful effect on wild and domestic animals as well as humans at low doses. Herein, we combine confocal Raman spectroscopy with SERS-AG substrate to characterize the "fingerprint" information of MC-LR directly. High sensitivity of SERS-AG substrates was verified by utilizing the probe molecule Rhodamine 6 G. Mapping spectra demonstrated good reproducibility of MC-LR identification with label-free surface-enhanced Raman scattering (SERS) strategy. Differences between SERS spectra of MC-LR and R6G, microcystin-RR were evaluated by calculating their scores and loading weights with an unsupervised exploratory principal component analysis method. Then, relationship between Raman intensities and concentrations was preliminary analyzed with SERS spectra of MC-LR and the lowest concentration of MC-LR identification was 10-6 mg L-1 while using SERS-AG substrate. Thereafter, 68.6% quantitative recovery of 10-3 mg L-1 MC-LR in tap water samples was obtained by the proposed label-free SERS method. These results showed that confocal Raman spectroscopy with label-free surface-enhanced Raman scattering strategy can handle the identification of trace MC-LR for monitoring water quality and safety worldwide in future.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:195 |
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| Enthalten in: |
Talanta - 195(2019) vom: 01. Apr., Seite 401-406 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
He, Shixuan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.02.2019 Date Revised 09.12.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.talanta.2018.11.072 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM292539673 |
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| 520 | |a Copyright © 2018 Elsevier B.V. All rights reserved. | ||
| 520 | |a The analysis of trace microcystin-LR (MC-LR) plays important roles in environmental fields, especially in monitoring domestic water quality and safety, since it has particularly harmful effect on wild and domestic animals as well as humans at low doses. Herein, we combine confocal Raman spectroscopy with SERS-AG substrate to characterize the "fingerprint" information of MC-LR directly. High sensitivity of SERS-AG substrates was verified by utilizing the probe molecule Rhodamine 6 G. Mapping spectra demonstrated good reproducibility of MC-LR identification with label-free surface-enhanced Raman scattering (SERS) strategy. Differences between SERS spectra of MC-LR and R6G, microcystin-RR were evaluated by calculating their scores and loading weights with an unsupervised exploratory principal component analysis method. Then, relationship between Raman intensities and concentrations was preliminary analyzed with SERS spectra of MC-LR and the lowest concentration of MC-LR identification was 10-6 mg L-1 while using SERS-AG substrate. Thereafter, 68.6% quantitative recovery of 10-3 mg L-1 MC-LR in tap water samples was obtained by the proposed label-free SERS method. These results showed that confocal Raman spectroscopy with label-free surface-enhanced Raman scattering strategy can handle the identification of trace MC-LR for monitoring water quality and safety worldwide in future | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Microcystin-LR | |
| 650 | 4 | |a Principal component analysis | |
| 650 | 4 | |a Surface-enhanced Raman scattering | |
| 650 | 7 | |a Drinking Water |2 NLM | |
| 650 | 7 | |a Fluorescent Dyes |2 NLM | |
| 650 | 7 | |a Marine Toxins |2 NLM | |
| 650 | 7 | |a Microcystins |2 NLM | |
| 650 | 7 | |a Rhodamines |2 NLM | |
| 650 | 7 | |a Water Pollutants, Chemical |2 NLM | |
| 650 | 7 | |a microcystin RR |2 NLM | |
| 650 | 7 | |a CZ021GH33H |2 NLM | |
| 650 | 7 | |a cyanoginosin LR |2 NLM | |
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| 700 | 1 | |a Xie, Wanyi |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Shaoxi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Daming |e verfasserin |4 aut | |
| 700 | 1 | |a Djebbi, Khouloud |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Zhiyou |e verfasserin |4 aut | |
| 700 | 1 | |a Du, Jinglei |e verfasserin |4 aut | |
| 700 | 1 | |a Du, Chunlei |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Deqiang |e verfasserin |4 aut | |
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