Developing disease-resistant thermosensitive male sterile rice by multiplex gene editing
© 2019 Institute of Botany, Chinese Academy of Sciences..
High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding. We introduced specific mutations into the TMS5, Pi21, and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system. We found that the transgene-free homozygous triple tms5/pi21/xa13 mutants obtained in the T1 generation displayed characteristics of thermosensitive genic male sterility (TGMS) with enhanced resistance to rice blast and bacterial blight. Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing, which could significantly accelerate the breeding of sterile lines.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2019 |
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Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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Enthalten in: |
Journal of integrative plant biology - 61(2019), 12 vom: 15. Dez., Seite 1201-1205 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Li, Sanfeng [VerfasserIn] |
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Links: |
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Themen: |
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Anmerkungen: |
Date Completed 04.05.2020 Date Revised 05.05.2020 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1111/jipb.12774 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM292520379 |
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