NZO/HlLtJ as a novel model for the studies on the role of metabolic syndrome in acute radiation toxicity
Purpose: Growing rates of metabolic syndrome and associated obesity warrant the development of appropriate animal models for better understanding of how those conditions may affect sensitivity to IR exposure.Materials and methods: We subjected male NZO/HlLtJ mice, a strain prone to spontaneous obesity and diabetes, to 0, 5.5, 6.37, 7.4 or 8.5 Gy (137Cs) of total body irradiation (TBI). Mice were monitored for 30 days, after which proximal jejunum and colon tissues were collected for further histological and molecular analysis.Results: Obese NZO/HlLtJ male mice are characterized by their lower sensitivity to IR at doses of 6.37 Gy and under, compared to other strains. Further escalation of the dose, however, results in a steep survival curve, reaching LD100/30 values at a dose of 8.5 Gy. Alterations in the expression of various tight junction-related proteins coupled with activation of inflammatory responses and cell death were the main contributors to the gastrointestinal syndrome.Conclusions: We demonstrate that metabolic syndrome with exhibited hyperglycemia but without alterations to the microvasculature is not a pre-requisite of the increased sensitivity to TBI at high doses. Our studies indicate the potential of NZO/HlLtJ mice for the studies on the role of metabolic syndrome in acute radiation toxicity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:96 |
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| Enthalten in: |
International journal of radiation biology - 96(2020), 1 vom: 17. Jan., Seite 93-99 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ewing, Laura E [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 30.01.2020 Date Revised 14.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1080/09553002.2018.1547437 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM291910025 |
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| 245 | 1 | 0 | |a NZO/HlLtJ as a novel model for the studies on the role of metabolic syndrome in acute radiation toxicity |
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| 500 | |a Date Revised 14.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Purpose: Growing rates of metabolic syndrome and associated obesity warrant the development of appropriate animal models for better understanding of how those conditions may affect sensitivity to IR exposure.Materials and methods: We subjected male NZO/HlLtJ mice, a strain prone to spontaneous obesity and diabetes, to 0, 5.5, 6.37, 7.4 or 8.5 Gy (137Cs) of total body irradiation (TBI). Mice were monitored for 30 days, after which proximal jejunum and colon tissues were collected for further histological and molecular analysis.Results: Obese NZO/HlLtJ male mice are characterized by their lower sensitivity to IR at doses of 6.37 Gy and under, compared to other strains. Further escalation of the dose, however, results in a steep survival curve, reaching LD100/30 values at a dose of 8.5 Gy. Alterations in the expression of various tight junction-related proteins coupled with activation of inflammatory responses and cell death were the main contributors to the gastrointestinal syndrome.Conclusions: We demonstrate that metabolic syndrome with exhibited hyperglycemia but without alterations to the microvasculature is not a pre-requisite of the increased sensitivity to TBI at high doses. Our studies indicate the potential of NZO/HlLtJ mice for the studies on the role of metabolic syndrome in acute radiation toxicity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Acute radiation gastrointestinal injury | |
| 650 | 4 | |a glucose metabolism | |
| 650 | 4 | |a metabolic syndrome | |
| 650 | 4 | |a obesity | |
| 650 | 4 | |a tight junction-related proteins | |
| 650 | 7 | |a Blood Glucose |2 NLM | |
| 700 | 1 | |a Miousse, Isabelle R |e verfasserin |4 aut | |
| 700 | 1 | |a Pathak, Rupak |e verfasserin |4 aut | |
| 700 | 1 | |a Skinner, Charles M |e verfasserin |4 aut | |
| 700 | 1 | |a Kosanke, Stanley |e verfasserin |4 aut | |
| 700 | 1 | |a Boerma, Marjan |e verfasserin |4 aut | |
| 700 | 1 | |a Hauer-Jensen, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Koturbash, Igor |e verfasserin |4 aut | |
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