Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro
Suicide gene therapy induced by the Herpes Simplex Virus thymidine kinase/ganciclovir (HSV-tk/GCV) system has been utilized to successfully treat various cancers. We describe TransfeX-mediated transfection of pCMV.Luc into HeLa cervical carcinoma and HSC-3, FaDu, and H357 oral squamous cell carcinoma (OSCC) cell lines in the presence of 10% serum. This method has proved to be highly efficient, with low nonspecific cytotoxicity. The plasmid pNGVL1-tk encoding HSV-tk under the control of the CMV promoter was delivered to the cells in vitro via TransfeX, a cationic liposomal reagent, followed by treatment with ganciclovir. The Alamar Blue cell viability assay was used to determine levels of the suicide effect.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1895 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 1895(2019) vom: 11., Seite 177-184 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Düzgüneş, Nejat [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 24.06.2019 Date Revised 24.06.2019 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-4939-8922-5_14 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM291696473 |
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| 520 | |a Suicide gene therapy induced by the Herpes Simplex Virus thymidine kinase/ganciclovir (HSV-tk/GCV) system has been utilized to successfully treat various cancers. We describe TransfeX-mediated transfection of pCMV.Luc into HeLa cervical carcinoma and HSC-3, FaDu, and H357 oral squamous cell carcinoma (OSCC) cell lines in the presence of 10% serum. This method has proved to be highly efficient, with low nonspecific cytotoxicity. The plasmid pNGVL1-tk encoding HSV-tk under the control of the CMV promoter was delivered to the cells in vitro via TransfeX, a cationic liposomal reagent, followed by treatment with ganciclovir. The Alamar Blue cell viability assay was used to determine levels of the suicide effect | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Oral squamous cell carcinoma | |
| 650 | 4 | |a Transfection | |
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| 700 | 1 | |a Cheung, Jennifer |e verfasserin |4 aut | |
| 700 | 1 | |a Konopka, Krystyna |e verfasserin |4 aut | |
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