Methylation profiles of IL33 and CCL26 in bronchial epithelial cells are associated with asthma
AIM: This study aimed to characterize DNA methylation (DNA-me) in promoter region of IL33, IL1RL1 and CCL26 in asthma and their impacts on transcriptional activity in bronchial epithelial cells (BECs).
PATIENTS & METHODS: We performed bis-pyrosequencing, quantitative real-time PCR and sequencing in BECs from ten asthmatic and ten control individuals.
RESULTS: We detected lower DNA-me levels of IL33 and CCL26 in asthmatic than control BECs. No correlation was found between methylation and expression levels. Interestingly, carriers of a mutative allele in a haplotype within the promoter of IL33 had a lower IL33 DNA-me level and CCL26 gene expression correlated with eosinophil count.
CONCLUSION: These findings highlight the importance of investigating both epigenetic and genetic mechanisms in understanding the epithelial immune response in asthma.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
Epigenomics - 10(2018), 12 vom: 11. Dez., Seite 1555-1568 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Larouche, Miriam [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 29.07.2019 Date Revised 29.07.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.2217/epi-2018-0044 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM290997992 |
|---|
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| 041 | |a eng | ||
| 100 | 1 | |a Larouche, Miriam |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Methylation profiles of IL33 and CCL26 in bronchial epithelial cells are associated with asthma |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 29.07.2019 | ||
| 500 | |a Date Revised 29.07.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a AIM: This study aimed to characterize DNA methylation (DNA-me) in promoter region of IL33, IL1RL1 and CCL26 in asthma and their impacts on transcriptional activity in bronchial epithelial cells (BECs) | ||
| 520 | |a PATIENTS & METHODS: We performed bis-pyrosequencing, quantitative real-time PCR and sequencing in BECs from ten asthmatic and ten control individuals | ||
| 520 | |a RESULTS: We detected lower DNA-me levels of IL33 and CCL26 in asthmatic than control BECs. No correlation was found between methylation and expression levels. Interestingly, carriers of a mutative allele in a haplotype within the promoter of IL33 had a lower IL33 DNA-me level and CCL26 gene expression correlated with eosinophil count | ||
| 520 | |a CONCLUSION: These findings highlight the importance of investigating both epigenetic and genetic mechanisms in understanding the epithelial immune response in asthma | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a DNA methylation | |
| 650 | 4 | |a bronchial epithelial cell | |
| 650 | 4 | |a eosinophil | |
| 650 | 4 | |a gene expression | |
| 650 | 7 | |a CCL26 protein, human |2 NLM | |
| 650 | 7 | |a Chemokine CCL26 |2 NLM | |
| 650 | 7 | |a IL1RL1 protein, human |2 NLM | |
| 650 | 7 | |a IL33 protein, human |2 NLM | |
| 650 | 7 | |a Interleukin-1 Receptor-Like 1 Protein |2 NLM | |
| 650 | 7 | |a Interleukin-33 |2 NLM | |
| 700 | 1 | |a Gagné-Ouellet, Valérie |e verfasserin |4 aut | |
| 700 | 1 | |a Boucher-Lafleur, Anne-Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Larose, Marie-Chantal |e verfasserin |4 aut | |
| 700 | 1 | |a Plante, Sophie |e verfasserin |4 aut | |
| 700 | 1 | |a Madore, Anne-Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Laviolette, Michel |e verfasserin |4 aut | |
| 700 | 1 | |a Flamand, Nicolas |e verfasserin |4 aut | |
| 700 | 1 | |a Chakir, Jamila |e verfasserin |4 aut | |
| 700 | 1 | |a Laprise, Catherine |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:10 |g year:2018 |g number:12 |g day:11 |g month:12 |g pages:1555-1568 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.2217/epi-2018-0044 |3 Volltext |
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