Autologous blood transfusion augments impaired wound healing in diabetic mice by enhancing lncRNA H19 expression via the HIF-1α signaling pathway
BACKGROUND: Impaired wound healing frequently occurs in diabetes mellitus (DM) and is implicated in impaired angiogenesis. Long non-coding RNA (lncRNA) H19 has been reported as being reduced in DM and played a critical role in inducing angiogenesis. Thus, we hypothesized that H19 may affect impaired wound healing in streptozotocin (STZ)-induced diabetic mice transfused with autologous blood preserved in standard preservative fluid or modified preservative fluid.
METHODS: Fibroblasts in injured skin were isolated and cultured in vitro. After location of H19 in fibroblasts using fluorescence in situ hybridization (FISH), RNA-pull down, RNA immunoprecipitation (RIP), chromatin immunoprecipitation (ChIP), Co immunoprecipitation (COIP) and dual luciferase reporter gene assay were used to verify the binding of H19 to HIF-1α.
RESULTS: The modified preservative fluid preserved autologous blood increased the H19 expression in fibroblasts, and maintained better oxygen-carrying and oxygen release capacities as well as coagulation function. Furthermore, H19 promoted HIF-1α histone H3K4me3 methylation and increased HIF-1α expression by recruiting EZH2. H19 promoted fibroblast activation by activating HIF-1α signaling pathway in fibroblasts and enhanced wound healing in diabetic mice.
CONCLUSIONS: Taken together, H19 accelerated fibroblast activation by recruiting EZH2-mediated histone methylation and modulating the HIF-1α signaling pathway, whereby augmenting the process of modified preservative fluid preserved autologous blood enhancing the postoperative wound healing in diabetic mice.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Cell communication and signaling : CCS - 16(2018), 1 vom: 20. Nov., Seite 84 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Guo, Jian-Rong [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 15.05.2019 Date Revised 15.05.2019 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s12964-018-0290-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM290904048 |
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| 041 | |a eng | ||
| 100 | 1 | |a Guo, Jian-Rong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Autologous blood transfusion augments impaired wound healing in diabetic mice by enhancing lncRNA H19 expression via the HIF-1α signaling pathway |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 15.05.2019 | ||
| 500 | |a Date Revised 15.05.2019 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Impaired wound healing frequently occurs in diabetes mellitus (DM) and is implicated in impaired angiogenesis. Long non-coding RNA (lncRNA) H19 has been reported as being reduced in DM and played a critical role in inducing angiogenesis. Thus, we hypothesized that H19 may affect impaired wound healing in streptozotocin (STZ)-induced diabetic mice transfused with autologous blood preserved in standard preservative fluid or modified preservative fluid | ||
| 520 | |a METHODS: Fibroblasts in injured skin were isolated and cultured in vitro. After location of H19 in fibroblasts using fluorescence in situ hybridization (FISH), RNA-pull down, RNA immunoprecipitation (RIP), chromatin immunoprecipitation (ChIP), Co immunoprecipitation (COIP) and dual luciferase reporter gene assay were used to verify the binding of H19 to HIF-1α | ||
| 520 | |a RESULTS: The modified preservative fluid preserved autologous blood increased the H19 expression in fibroblasts, and maintained better oxygen-carrying and oxygen release capacities as well as coagulation function. Furthermore, H19 promoted HIF-1α histone H3K4me3 methylation and increased HIF-1α expression by recruiting EZH2. H19 promoted fibroblast activation by activating HIF-1α signaling pathway in fibroblasts and enhanced wound healing in diabetic mice | ||
| 520 | |a CONCLUSIONS: Taken together, H19 accelerated fibroblast activation by recruiting EZH2-mediated histone methylation and modulating the HIF-1α signaling pathway, whereby augmenting the process of modified preservative fluid preserved autologous blood enhancing the postoperative wound healing in diabetic mice | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Diabetes mellitus mice | |
| 650 | 4 | |a EZH2 | |
| 650 | 4 | |a Fibroblast activation | |
| 650 | 4 | |a HIF-1α signaling pathway | |
| 650 | 4 | |a Histone methylation | |
| 650 | 4 | |a Long non-coding RNA H19 | |
| 650 | 4 | |a Modified autologous blood | |
| 650 | 4 | |a Wound healing | |
| 650 | 7 | |a H19 long non-coding RNA |2 NLM | |
| 650 | 7 | |a Histones |2 NLM | |
| 650 | 7 | |a Hypoxia-Inducible Factor 1, alpha Subunit |2 NLM | |
| 650 | 7 | |a RNA, Long Noncoding |2 NLM | |
| 650 | 7 | |a Enhancer of Zeste Homolog 2 Protein |2 NLM | |
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| 650 | 7 | |a Ezh2 protein, mouse |2 NLM | |
| 650 | 7 | |a EC 2.1.1.43 |2 NLM | |
| 700 | 1 | |a Yin, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yong-Quan |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Xiao-Ju |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Xun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Na-Na |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiao-Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Han-Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Duan, Li-Shuang |e verfasserin |4 aut | |
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