Testing bio-efficacy of insecticide-treated nets with fewer mosquitoes for enhanced malaria control
Malaria control programs implementing Long-Lasting Insecticidal Nets (LLINs) are encouraged to conduct field monitoring of nets' survival, fabric integrity and insecticidal bio-efficacy. The reference method for testing the insecticide activity of LLINs needs 100 two-to-five-day-old female mosquitoes per net, which is highly resource-intensive. We aimed at identifying an alternative protocol, using fewer mosquitos, while ensuring a precision in the main indicator of ±5 percentage points (pp). We compared different laboratory methods against the probability of the LLIN to fail the test as determined by a hierarchical Bayesian model. When using 50 mosquitoes per LLIN and considering mortality only instead of mortality or knock-down as validity criteria, the average error in the measure of the proportion of nets considered as valid was 0.40 pp. The 95% confidence interval of this value never exceed 5 pp when the number of LLIN tested was ≥40. This method slightly outperforms the current recommendations. As a conclusion, testing the bio-efficacy of LLINs with half as many mosquitoes provides a valid evaluation of the proportion of valid LLINs. This approach could increase entomology labs' testing capacity and decrease costs, with no impact in the decision process for public health purposes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
Scientific reports - 8(2018), 1 vom: 13. Nov., Seite 16769 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Boyer, Sebastien [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Completed 01.11.2019 Date Revised 13.11.2019 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-018-34979-3 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM290576334 |
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| 520 | |a Malaria control programs implementing Long-Lasting Insecticidal Nets (LLINs) are encouraged to conduct field monitoring of nets' survival, fabric integrity and insecticidal bio-efficacy. The reference method for testing the insecticide activity of LLINs needs 100 two-to-five-day-old female mosquitoes per net, which is highly resource-intensive. We aimed at identifying an alternative protocol, using fewer mosquitos, while ensuring a precision in the main indicator of ±5 percentage points (pp). We compared different laboratory methods against the probability of the LLIN to fail the test as determined by a hierarchical Bayesian model. When using 50 mosquitoes per LLIN and considering mortality only instead of mortality or knock-down as validity criteria, the average error in the measure of the proportion of nets considered as valid was 0.40 pp. The 95% confidence interval of this value never exceed 5 pp when the number of LLIN tested was ≥40. This method slightly outperforms the current recommendations. As a conclusion, testing the bio-efficacy of LLINs with half as many mosquitoes provides a valid evaluation of the proportion of valid LLINs. This approach could increase entomology labs' testing capacity and decrease costs, with no impact in the decision process for public health purposes | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Pothin, Emilie |e verfasserin |4 aut | |
| 700 | 1 | |a Randriamaherijaona, Sanjiarizaha |e verfasserin |4 aut | |
| 700 | 1 | |a Rogier, Christophe |e verfasserin |4 aut | |
| 700 | 1 | |a Kesteman, Thomas |e verfasserin |4 aut | |
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