Beyond O6-Benzylguanine : O6-(5-Pyridylmethyl)guanine as a Substrate for the Self-Labeling Enzyme SNAP-Tag
SNAP-tag is a genetically encoded label for tracking proteins of interest that is known to utilize O6-benzylguanine derivatives as substrates. In this work, mass spectrometric analysis revealed that SNAP-tag also accepts O6-(5-pyridylmethyl)guanine derivatives as substrates. A fluorescently conjugated O6-(5-pyridylmethyl)guanine was synthesized and used to selectively label intracellular compartments. This compound also acts as a zinc(II) ion-sensitive fluorescent indicator and therefore can be used in organelle-specific zinc(II) imaging.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
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| Enthalten in: |
Bioconjugate chemistry - 29(2018), 12 vom: 19. Dez., Seite 4104-4109 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Macias-Contreras, Miguel [VerfasserIn] |
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| Links: |
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| Themen: |
01KC87F8FE |
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| Anmerkungen: |
Date Completed 13.08.2019 Date Revised 13.08.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.bioconjchem.8b00703 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM290446120 |
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| 520 | |a SNAP-tag is a genetically encoded label for tracking proteins of interest that is known to utilize O6-benzylguanine derivatives as substrates. In this work, mass spectrometric analysis revealed that SNAP-tag also accepts O6-(5-pyridylmethyl)guanine derivatives as substrates. A fluorescently conjugated O6-(5-pyridylmethyl)guanine was synthesized and used to selectively label intracellular compartments. This compound also acts as a zinc(II) ion-sensitive fluorescent indicator and therefore can be used in organelle-specific zinc(II) imaging | ||
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