Epigallocatechin-3-gallate Enhances Radiation Sensitivity in Colorectal Cancer Cells Through Nrf2 Activation and Autophagy
Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved..
BACKGROUND/AIM: Epigallocatechin-3-gallate (EGCG) is a major polyphenolic component of green tea. EGCG plays a potential role in radio-sensitizing cancer cells. The combined effect of EGCG and radiation was investigated in a colorectal cancer cell line, focusing on nuclear factor (erythroid-derived 2)-like 2 (Nrf2) autophagy signalling.
MATERIALS AND METHODS: HCT-116 cells were treated with 12.5 μM EGCG for different periods of time, 2 Gy radiation, or both. Cell viability was determined with the WST-8 assay. The number of colonies was determined with the colony formation assay. mRNA expression of LC3 and caspase-9 was analyzed with quantitative real-time polymerase chain reaction.
RESULTS: Combination treatment with EGCG and radiation significantly decreased the growth of HCT-116 cells. The number of colonies was reduced to 34.2% compared to the control group. Immunofluorescence microscopy images showed that nuclear translocation of Nrf2 was significantly increased when cells were treated with the combination of EGCG and radiation compared to the control and single-treatment groups. Combined treatment with EGCG and radiation significantly induced LC3 and caspase-9 mRNA expression.
CONCLUSION: EGCG increased the sensitivity of colorectal cancer cells to radiation by inhibiting cell proliferation and inducing Nrf2 nuclear translocation and autophagy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:38 |
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| Enthalten in: |
Anticancer research - 38(2018), 11 vom: 02. Nov., Seite 6247-6252 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Enkhbat, Tumenjin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 08.11.2018 Date Revised 17.06.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.21873/anticanres.12980 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM290302021 |
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| 245 | 1 | 0 | |a Epigallocatechin-3-gallate Enhances Radiation Sensitivity in Colorectal Cancer Cells Through Nrf2 Activation and Autophagy |
| 264 | 1 | |c 2018 | |
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| 500 | |a Date Completed 08.11.2018 | ||
| 500 | |a Date Revised 17.06.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. | ||
| 520 | |a BACKGROUND/AIM: Epigallocatechin-3-gallate (EGCG) is a major polyphenolic component of green tea. EGCG plays a potential role in radio-sensitizing cancer cells. The combined effect of EGCG and radiation was investigated in a colorectal cancer cell line, focusing on nuclear factor (erythroid-derived 2)-like 2 (Nrf2) autophagy signalling | ||
| 520 | |a MATERIALS AND METHODS: HCT-116 cells were treated with 12.5 μM EGCG for different periods of time, 2 Gy radiation, or both. Cell viability was determined with the WST-8 assay. The number of colonies was determined with the colony formation assay. mRNA expression of LC3 and caspase-9 was analyzed with quantitative real-time polymerase chain reaction | ||
| 520 | |a RESULTS: Combination treatment with EGCG and radiation significantly decreased the growth of HCT-116 cells. The number of colonies was reduced to 34.2% compared to the control group. Immunofluorescence microscopy images showed that nuclear translocation of Nrf2 was significantly increased when cells were treated with the combination of EGCG and radiation compared to the control and single-treatment groups. Combined treatment with EGCG and radiation significantly induced LC3 and caspase-9 mRNA expression | ||
| 520 | |a CONCLUSION: EGCG increased the sensitivity of colorectal cancer cells to radiation by inhibiting cell proliferation and inducing Nrf2 nuclear translocation and autophagy | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Nishi, Masaaki |e verfasserin |4 aut | |
| 700 | 1 | |a Yoshikawa, Kozo |e verfasserin |4 aut | |
| 700 | 1 | |a Jun, Higashijima |e verfasserin |4 aut | |
| 700 | 1 | |a Tokunaga, Takuya |e verfasserin |4 aut | |
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| 700 | 1 | |a Ishikawa, Daichi |e verfasserin |4 aut | |
| 700 | 1 | |a Tominaga, Masahide |e verfasserin |4 aut | |
| 700 | 1 | |a Shimada, Mitsuo |e verfasserin |4 aut | |
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