Preparative separation of caffeoylquinic acid isomers from Lonicerae japonicae Flos by pH-zone-refining counter-current chromatography and a strategy for selection of solvent systems with high sample loading capacities
Copyright © 2018 Elsevier B.V. All rights reserved..
Caffeoylquinic acid derivatives exhibit anti-inflammatory, antioxidant, and antibacterial activities. We successfully applied pH-zone-refining counter-current chromatography (pH-ZRCCC) to separation of isomeric caffeoylquinic acids from Lonicerae japonicae Flos using a two-phase solvent system composed of ethyl acetate-n-butanol-acetonitrile-water (3:1:1:5, v/v/v/v). Trifluoroacetic acid (10 mM) was added to the upper phase as a retainer and ammonium hydroxide (10 mM) was added to the lower phase as an eluter. As a result, 167.8 mg of chlorogenic acid, 15.9 mg of isochlorogenic acid B, 103.4 mg of isochlorogenic acid C, and 156.0 mg of isochlorogenic acid A were obtained from 1.2 g of crude extract, and all compound purities were over 96%. In addition, by comparing the numeric values of partition coefficient of compounds, it was found that larger differences between the K values of adjacent compounds in the solvent systems resulted in higher sample loading capacities. pH-ZRCCC method is an efficient preparative separation of isomeric caffeoylquinic acids from natural products.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1578 |
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| Enthalten in: |
Journal of chromatography. A - 1578(2018) vom: 30. Nov., Seite 61-66 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ma, Tianyu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.12.2018 Date Revised 04.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.chroma.2018.10.014 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM29000716X |
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| 245 | 1 | 0 | |a Preparative separation of caffeoylquinic acid isomers from Lonicerae japonicae Flos by pH-zone-refining counter-current chromatography and a strategy for selection of solvent systems with high sample loading capacities |
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| 520 | |a Copyright © 2018 Elsevier B.V. All rights reserved. | ||
| 520 | |a Caffeoylquinic acid derivatives exhibit anti-inflammatory, antioxidant, and antibacterial activities. We successfully applied pH-zone-refining counter-current chromatography (pH-ZRCCC) to separation of isomeric caffeoylquinic acids from Lonicerae japonicae Flos using a two-phase solvent system composed of ethyl acetate-n-butanol-acetonitrile-water (3:1:1:5, v/v/v/v). Trifluoroacetic acid (10 mM) was added to the upper phase as a retainer and ammonium hydroxide (10 mM) was added to the lower phase as an eluter. As a result, 167.8 mg of chlorogenic acid, 15.9 mg of isochlorogenic acid B, 103.4 mg of isochlorogenic acid C, and 156.0 mg of isochlorogenic acid A were obtained from 1.2 g of crude extract, and all compound purities were over 96%. In addition, by comparing the numeric values of partition coefficient of compounds, it was found that larger differences between the K values of adjacent compounds in the solvent systems resulted in higher sample loading capacities. pH-ZRCCC method is an efficient preparative separation of isomeric caffeoylquinic acids from natural products | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Caffeoylquinic acid isomer | |
| 650 | 4 | |a Lonicerae japonicae Flos | |
| 650 | 4 | |a Preparative separation | |
| 650 | 4 | |a Sample loading capacity | |
| 650 | 4 | |a pH-Zone-refining counter-current chromatography | |
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| 650 | 7 | |a Plant Extracts |2 NLM | |
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| 650 | 7 | |a isochlorogenic acid |2 NLM | |
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| 650 | 7 | |a 318ADP12RI |2 NLM | |
| 650 | 7 | |a lonicerae flos |2 NLM | |
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| 650 | 7 | |a acetonitrile |2 NLM | |
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| 700 | 1 | |a Dong, Hongjing |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Heng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Hengqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Lanping |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiao |e verfasserin |4 aut | |
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