Genetic deletion of the alamandine receptor MRGD leads to dilated cardiomyopathy in mice
We have recently described a new peptide of the renin-angiotensin system, alamandine, a derivative of angiotensin-(1-7). Mas-related G protein-coupled receptor member D (MrgD) was identified as its receptor. Although similar cardioprotective effects of alamandine to those of angiotensin-(1-7) have been described, the significance of this peptide in heart function is still elusive. We aimed to evaluate the functional role of the alamandine receptor MrgD in the heart using MrgD-deficient mice. MrgD was localized in cardiomyocytes by immunofluorescence using confocal microscopy. High-resolution echocardiography was performed in wild-type and MrgD-deficient mice (2 and 12 wk old) under isoflurane anesthesia. Standard B-mode images were obtained in the right and left parasternal long and short axes for morphological and functional assessment and evaluation of cardiac deformation. Additional heart function evaluation was performed using Langendorff isolated heart preparations and inotropic measurements of isolated cardiomyocytes. Immunofluorescence indicated that the MrgD receptor is expressed in cardiomyocytes, mainly in the membrane and perinuclear and nuclear regions. Echocardiography showed left ventricular remodeling and severe dysfunction in MrgD-deficient mice. Strikingly, MrgD-deficient mice presented a pronounced dilated cardiomyopathy with a marked decrease in systolic function. Echocardiographic changes were supported by the data obtained in isolated hearts and inotropic measurements in cardiomyocytes. Our data add new evidence for a major role for alamandine/MrgD in the heart. Furthermore, our results indicate that we have identified a new gene implicated in dilated cardiomyopathy, unveiling a new target for translational approaches aimed to treat heart diseases. NEW & NOTEWORTHY The renin-angiotensin system is a key target for cardiovascular therapy. We have recently identified a new vasodepressor/cardioprotective angiotensin, alamandine. Here, we unmasked a key role for its receptor, Mas-related G protein-coupled receptor member D (MrgD), in heart function. The severe dilated cardiomyopathy observed in MrgD-deficient mice warrants clinical and preclinical studies to unveil its potential use in cardiovascular therapy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:316 |
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| Enthalten in: |
American journal of physiology. Heart and circulatory physiology - 316(2019), 1 vom: 01. Jan., Seite H123-H133 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Oliveira, Aline Cristina [VerfasserIn] |
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| Links: |
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| Themen: |
Alamandine |
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| Anmerkungen: |
Date Completed 14.11.2019 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1152/ajpheart.00075.2018 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Oliveira, Aline Cristina |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genetic deletion of the alamandine receptor MRGD leads to dilated cardiomyopathy in mice |
| 264 | 1 | |c 2019 | |
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| 500 | |a Date Revised 30.09.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We have recently described a new peptide of the renin-angiotensin system, alamandine, a derivative of angiotensin-(1-7). Mas-related G protein-coupled receptor member D (MrgD) was identified as its receptor. Although similar cardioprotective effects of alamandine to those of angiotensin-(1-7) have been described, the significance of this peptide in heart function is still elusive. We aimed to evaluate the functional role of the alamandine receptor MrgD in the heart using MrgD-deficient mice. MrgD was localized in cardiomyocytes by immunofluorescence using confocal microscopy. High-resolution echocardiography was performed in wild-type and MrgD-deficient mice (2 and 12 wk old) under isoflurane anesthesia. Standard B-mode images were obtained in the right and left parasternal long and short axes for morphological and functional assessment and evaluation of cardiac deformation. Additional heart function evaluation was performed using Langendorff isolated heart preparations and inotropic measurements of isolated cardiomyocytes. Immunofluorescence indicated that the MrgD receptor is expressed in cardiomyocytes, mainly in the membrane and perinuclear and nuclear regions. Echocardiography showed left ventricular remodeling and severe dysfunction in MrgD-deficient mice. Strikingly, MrgD-deficient mice presented a pronounced dilated cardiomyopathy with a marked decrease in systolic function. Echocardiographic changes were supported by the data obtained in isolated hearts and inotropic measurements in cardiomyocytes. Our data add new evidence for a major role for alamandine/MrgD in the heart. Furthermore, our results indicate that we have identified a new gene implicated in dilated cardiomyopathy, unveiling a new target for translational approaches aimed to treat heart diseases. NEW & NOTEWORTHY The renin-angiotensin system is a key target for cardiovascular therapy. We have recently identified a new vasodepressor/cardioprotective angiotensin, alamandine. Here, we unmasked a key role for its receptor, Mas-related G protein-coupled receptor member D (MrgD), in heart function. The severe dilated cardiomyopathy observed in MrgD-deficient mice warrants clinical and preclinical studies to unveil its potential use in cardiovascular therapy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Mas-related G protein-coupled receptor member D | |
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| 700 | 1 | |a Melo, Marcos Barrouin |e verfasserin |4 aut | |
| 700 | 1 | |a Motta-Santos, Daisy |e verfasserin |4 aut | |
| 700 | 1 | |a Peluso, A Augusto |e verfasserin |4 aut | |
| 700 | 1 | |a Souza-Neto, Fernando |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva, Rafaela F |e verfasserin |4 aut | |
| 700 | 1 | |a Almeida, Jonathas F Q |e verfasserin |4 aut | |
| 700 | 1 | |a Canta, Giovanni |e verfasserin |4 aut | |
| 700 | 1 | |a Reis, Adelina M |e verfasserin |4 aut | |
| 700 | 1 | |a Goncalves, Gleisy |e verfasserin |4 aut | |
| 700 | 1 | |a Cerri, Gabriela |e verfasserin |4 aut | |
| 700 | 1 | |a Coutinho, Danielle |e verfasserin |4 aut | |
| 700 | 1 | |a Guedes de Jesus, Itamar Couto |e verfasserin |4 aut | |
| 700 | 1 | |a Guatimosim, Silvia |e verfasserin |4 aut | |
| 700 | 1 | |a Linhares, Natalia D |e verfasserin |4 aut | |
| 700 | 1 | |a Alenina, Natalia |e verfasserin |4 aut | |
| 700 | 1 | |a Bader, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Campagnole-Santos, Maria José |e verfasserin |4 aut | |
| 700 | 1 | |a Santos, Robson A Souza |e verfasserin |4 aut | |
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