Combined use of Quantiferon and HBHA-based IGRA supports tuberculosis diagnosis and therapy management in children
Copyright © 2018 The British Infection Association. Published by Elsevier Ltd. All rights reserved..
OBJECTIVES: Interferon-γ release assays (IGRA) are designed for diagnosis of tuberculosis (TB) infection, and do not discriminate latent TB infection (LTBI) from active TB. Heparin-binding hemagglutinin antigen (HBHA) emerged as a promising antigen for TB diagnosis when used in IGRA format. Aim of this study was to prospectively evaluate the performance of an HBHA-based IGRA to support TB diagnosis and TB therapy monitoring in children with TB infection or active TB disease.
METHODS: Following clinical, microbiological and radiological assessment, children (0-14 years old) were tested by the QuantiFERON TB-Gold In tube (QFT) assay and an aliquot of whole-blood was stimulated with HBHA and IFNγ evaluated only in QFT-positive subjects.
RESULTS: Among the 550 children tested, 486 (88.4%) scored negative and 64 (11.6%) positive. None of the QFT-negative had active TB. Among the QFT-positive, 45 were with LTBI and 19 active TB. HBHA-IGRA scored positive in 41/45 children (91.1%) with LTBI and in 6/19 active TB children (31.6%) at diagnosis (p = 0.001); remarkably, 5 of these 6 children with active TB scoring HBHA-positive were asymptomatic. Moreover, following TB-specific therapy, most of the non-HBHA-responding children, gained an HBHA-positive response.
CONCLUSIONS: HBHA-based IGRA is a useful support in TB diagnosis and TB-therapy monitoring in children.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:77 |
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| Enthalten in: |
The Journal of infection - 77(2018), 6 vom: 01. Dez., Seite 526-533 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sali, Michela [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 27.09.2019 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jinf.2018.09.011 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM289035562 |
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| 245 | 1 | 0 | |a Combined use of Quantiferon and HBHA-based IGRA supports tuberculosis diagnosis and therapy management in children |
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| 500 | |a Date Completed 27.09.2019 | ||
| 500 | |a Date Revised 10.12.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 The British Infection Association. Published by Elsevier Ltd. All rights reserved. | ||
| 520 | |a OBJECTIVES: Interferon-γ release assays (IGRA) are designed for diagnosis of tuberculosis (TB) infection, and do not discriminate latent TB infection (LTBI) from active TB. Heparin-binding hemagglutinin antigen (HBHA) emerged as a promising antigen for TB diagnosis when used in IGRA format. Aim of this study was to prospectively evaluate the performance of an HBHA-based IGRA to support TB diagnosis and TB therapy monitoring in children with TB infection or active TB disease | ||
| 520 | |a METHODS: Following clinical, microbiological and radiological assessment, children (0-14 years old) were tested by the QuantiFERON TB-Gold In tube (QFT) assay and an aliquot of whole-blood was stimulated with HBHA and IFNγ evaluated only in QFT-positive subjects | ||
| 520 | |a RESULTS: Among the 550 children tested, 486 (88.4%) scored negative and 64 (11.6%) positive. None of the QFT-negative had active TB. Among the QFT-positive, 45 were with LTBI and 19 active TB. HBHA-IGRA scored positive in 41/45 children (91.1%) with LTBI and in 6/19 active TB children (31.6%) at diagnosis (p = 0.001); remarkably, 5 of these 6 children with active TB scoring HBHA-positive were asymptomatic. Moreover, following TB-specific therapy, most of the non-HBHA-responding children, gained an HBHA-positive response | ||
| 520 | |a CONCLUSIONS: HBHA-based IGRA is a useful support in TB diagnosis and TB-therapy monitoring in children | ||
| 650 | 4 | |a Evaluation Study | |
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Biomarkers | |
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| 700 | 1 | |a D'Alfonso, Pamela |e verfasserin |4 aut | |
| 700 | 1 | |a De Maio, Flavio |e verfasserin |4 aut | |
| 700 | 1 | |a Ceccarelli, Manuela |e verfasserin |4 aut | |
| 700 | 1 | |a Battah, Basem |e verfasserin |4 aut | |
| 700 | 1 | |a Palucci, Ivana |e verfasserin |4 aut | |
| 700 | 1 | |a Chiacchio, Teresa |e verfasserin |4 aut | |
| 700 | 1 | |a Goletti, Delia |e verfasserin |4 aut | |
| 700 | 1 | |a Sanguinetti, Maurizio |e verfasserin |4 aut | |
| 700 | 1 | |a Valentini, Piero |e verfasserin |4 aut | |
| 700 | 1 | |a Delogu, Giovanni |e verfasserin |4 aut | |
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