ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrprd6 mouse
The knockout (KO) of the adiponectin receptor 1 (AdipoR1) gene causes retinal degeneration. Here we report that ADIPOR1 protein is primarily found in the eye and brain with little expression in other tissues. Further analysis of AdipoR1 KO mice revealed that these animals exhibit early visual system abnormalities and are depleted of RHODOPSIN prior to pronounced photoreceptor death. A KO of AdipoR1 post-development either in photoreceptors or the retinal pigment epithelium (RPE) resulted in decreased expression of retinal proteins, establishing a role for ADIPOR1 in supporting vision in adulthood. Subsequent analysis of the Mfrprd6 mouse retina demonstrated that these mice are lacking ADIPOR1 in their RPE layer alone, suggesting that loss of ADIPOR1 drives retinal degeneration in this model. Moreover, we found elevated levels of IRBP in both the AdipoR1 KO and the Mfrprd6 models. The spatial distribution of IRBP was also abnormal. This dysregulation of IRBP hypothesizes a role for ADIPOR1 in retinoid metabolism.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
Scientific reports - 8(2018), 1 vom: 25. Sept., Seite 14339 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sluch, Valentin M [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 30.10.2019 Date Revised 13.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-018-32579-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM288903110 |
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| 100 | 1 | |a Sluch, Valentin M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrprd6 mouse |
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| 500 | |a Date Revised 13.03.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The knockout (KO) of the adiponectin receptor 1 (AdipoR1) gene causes retinal degeneration. Here we report that ADIPOR1 protein is primarily found in the eye and brain with little expression in other tissues. Further analysis of AdipoR1 KO mice revealed that these animals exhibit early visual system abnormalities and are depleted of RHODOPSIN prior to pronounced photoreceptor death. A KO of AdipoR1 post-development either in photoreceptors or the retinal pigment epithelium (RPE) resulted in decreased expression of retinal proteins, establishing a role for ADIPOR1 in supporting vision in adulthood. Subsequent analysis of the Mfrprd6 mouse retina demonstrated that these mice are lacking ADIPOR1 in their RPE layer alone, suggesting that loss of ADIPOR1 drives retinal degeneration in this model. Moreover, we found elevated levels of IRBP in both the AdipoR1 KO and the Mfrprd6 models. The spatial distribution of IRBP was also abnormal. This dysregulation of IRBP hypothesizes a role for ADIPOR1 in retinoid metabolism | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 7 | |a ADIPOR1 protein, human |2 NLM | |
| 650 | 7 | |a Eye Proteins |2 NLM | |
| 650 | 7 | |a Receptors, Adiponectin |2 NLM | |
| 650 | 7 | |a Retinoids |2 NLM | |
| 650 | 7 | |a Retinol-Binding Proteins |2 NLM | |
| 650 | 7 | |a adiponectin receptor 1, mouse |2 NLM | |
| 650 | 7 | |a interstitial retinol-binding protein |2 NLM | |
| 700 | 1 | |a Banks, Angela |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Crowley, Maura A |e verfasserin |4 aut | |
| 700 | 1 | |a Davis, Vanessa |e verfasserin |4 aut | |
| 700 | 1 | |a Xiang, Chuanxi |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Junzheng |e verfasserin |4 aut | |
| 700 | 1 | |a Demirs, John T |e verfasserin |4 aut | |
| 700 | 1 | |a Vrouvlianis, Joanna |e verfasserin |4 aut | |
| 700 | 1 | |a Leehy, Barrett |e verfasserin |4 aut | |
| 700 | 1 | |a Hanks, Shawn |e verfasserin |4 aut | |
| 700 | 1 | |a Hyman, Alexandra M |e verfasserin |4 aut | |
| 700 | 1 | |a Aranda, Jorge |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Bigelow, Chad E |e verfasserin |4 aut | |
| 700 | 1 | |a Rice, Dennis S |e verfasserin |4 aut | |
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