Imaging and Quantitative Detection of Lipid Droplets by Yellow Fluorescent Probes in Liver Sections of Plasmodium Infected Mice and Third Stage Human Cervical Cancer Tissues
The diagnosis and prognosis of the disease associated with lipid irregularity are areas of extreme significance. In this direction, fluoranthene based yellow fluorescent probes (FLUN-550, FLUN-552, FLUN-547) were designed and synthesized by conjugating the ethanolamine headgroup of the phospholipid phosphatidyl-ethanolamine present in biological membranes. Owing to unique photophysical properties and aqueous compatibility, these probes were successfully employed for staining lipid droplets (LDs) in preadipocytes and Leishmania donovani promastigotes. Furthermore, using the fluorescent probes FLUN-550 and FLUN-552 we successfully imaged and quantitatively detected the excess accumulation of lipids in a liver section of Plasmodium yoelii MDR infected mice (3- to 4-fold) and the tissue sections of third stage human cervical cancer patients (1.5- to 2-fold) compared to normal tissues. To the best of our knowledge, this is the first report of yellow fluorescent probes for imaging and quantitative detection of LDs in human cervical cancer tissues. These new yellow fluorescent lipid probes (FLUN-550 and FLUN-552) showed great potential for diagnosis of cervical cancer patients.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
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| Enthalten in: |
Bioconjugate chemistry - 29(2018), 11 vom: 21. Nov., Seite 3606-3613 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sharma, Ashutosh [VerfasserIn] |
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| Links: |
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| Themen: |
Fluorescent Dyes |
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| Anmerkungen: |
Date Completed 26.08.2019 Date Revised 26.08.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.bioconjchem.8b00552 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM288842448 |
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| 500 | |a Date Revised 26.08.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The diagnosis and prognosis of the disease associated with lipid irregularity are areas of extreme significance. In this direction, fluoranthene based yellow fluorescent probes (FLUN-550, FLUN-552, FLUN-547) were designed and synthesized by conjugating the ethanolamine headgroup of the phospholipid phosphatidyl-ethanolamine present in biological membranes. Owing to unique photophysical properties and aqueous compatibility, these probes were successfully employed for staining lipid droplets (LDs) in preadipocytes and Leishmania donovani promastigotes. Furthermore, using the fluorescent probes FLUN-550 and FLUN-552 we successfully imaged and quantitatively detected the excess accumulation of lipids in a liver section of Plasmodium yoelii MDR infected mice (3- to 4-fold) and the tissue sections of third stage human cervical cancer patients (1.5- to 2-fold) compared to normal tissues. To the best of our knowledge, this is the first report of yellow fluorescent probes for imaging and quantitative detection of LDs in human cervical cancer tissues. These new yellow fluorescent lipid probes (FLUN-550 and FLUN-552) showed great potential for diagnosis of cervical cancer patients | ||
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| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a Jha, Ajay K |e verfasserin |4 aut | |
| 700 | 1 | |a Mishra, Shachi |e verfasserin |4 aut | |
| 700 | 1 | |a Jain, Ankita |e verfasserin |4 aut | |
| 700 | 1 | |a Chauhan, Bhavana S |e verfasserin |4 aut | |
| 700 | 1 | |a Kathuria, Manoj |e verfasserin |4 aut | |
| 700 | 1 | |a Rawat, Kundan S |e verfasserin |4 aut | |
| 700 | 1 | |a Gupta, Neeraj M |e verfasserin |4 aut | |
| 700 | 1 | |a Tripathi, Renu |e verfasserin |4 aut | |
| 700 | 1 | |a Mitra, Kalyan |e verfasserin |4 aut | |
| 700 | 1 | |a Sachdev, Monika |e verfasserin |4 aut | |
| 700 | 1 | |a Bhatt, Madan L B |e verfasserin |4 aut | |
| 700 | 1 | |a Goel, Atul |e verfasserin |4 aut | |
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