Spectroscopic Study on the Interaction of Human Cytoglobin with Copper(Ⅱ) Ion
Cytoglobin (Cygb), a recently discovered member of the vertebrate globin family, exhibits a traditional globin fold with a three-over-three α-helical sandwich. The interaction between copper(Ⅱ) ion (Cu2+) and Cygb has been investigated by UV-Vis, fluorescence, synchronous fluorescence and circular dichroism (CD) spectra. Results showed that the absorption intensity of Cygb at 280 nm increased and the intrinsic fluorescence of Cygb was quenched when Cu2+ was added. This fluorescence quenching of Cygb has been proven that it belongs to static quenching. The synchronous fluorescence spectra indicated that there were small changes about the microenvironment of tryptophan residues and tyrosine residues; furthermore, the binding site of Cu2+ is closer to tryptophan residues than tyrosine residues. No obvious change was observed about the secondary structure of Cygb with the addition of Cu2+ from the CD spectra.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
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| Enthalten in: |
Guang pu xue yu guang pu fen xi = Guang pu - 37(2017), 1 vom: 10. Jan., Seite 321-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Jing-hong [VerfasserIn] |
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| Themen: |
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| Anmerkungen: |
Date Completed 04.02.2019 Date Revised 15.02.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM288591097 |
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| 245 | 1 | 0 | |a Spectroscopic Study on the Interaction of Human Cytoglobin with Copper(Ⅱ) Ion |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Cytoglobin (Cygb), a recently discovered member of the vertebrate globin family, exhibits a traditional globin fold with a three-over-three α-helical sandwich. The interaction between copper(Ⅱ) ion (Cu2+) and Cygb has been investigated by UV-Vis, fluorescence, synchronous fluorescence and circular dichroism (CD) spectra. Results showed that the absorption intensity of Cygb at 280 nm increased and the intrinsic fluorescence of Cygb was quenched when Cu2+ was added. This fluorescence quenching of Cygb has been proven that it belongs to static quenching. The synchronous fluorescence spectra indicated that there were small changes about the microenvironment of tryptophan residues and tyrosine residues; furthermore, the binding site of Cu2+ is closer to tryptophan residues than tyrosine residues. No obvious change was observed about the secondary structure of Cygb with the addition of Cu2+ from the CD spectra | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 7 | |a Copper |2 NLM | |
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| 700 | 1 | |a Bian, Lin |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Su-yan |e verfasserin |4 aut | |
| 700 | 1 | |a Kong, Jin-ming |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Lian-zhi |e verfasserin |4 aut | |
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