Releasable and traceless PEGylation of arginine-rich antimicrobial peptides
Arginine-rich antimicrobial peptides (AMPs) are emerging therapeutics of interest. However, their applicability is limited by their short circulation half-life, caused in part by their small size and digestion by blood proteases. This study reports a strategy to temporarily mask arginine residues within AMPs with methoxy poly(ethylene glycol). Based on the reagent used, release of AMPs occurred in hours to days in a completely traceless fashion. In vitro, conjugates were insensitive to serum proteases, and released native AMP with full in vitro bioactivity. This strategy is thus highly relevant and should be adaptable to the entire family of arginine-rich AMPs. It may potentially be used to improve AMP-therapies by providing a more steady concentration of AMP in the blood after a single injection, avoiding toxic effects at high AMP doses, and reducing the number of doses required over the treatment duration.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2017 |
---|---|
Erschienen: |
2017 |
Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
---|---|
Enthalten in: |
Chemical science - 8(2017), 5 vom: 01. Mai, Seite 4082-4086 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Gong, Y [VerfasserIn] |
---|
Links: |
---|
Themen: |
---|
Anmerkungen: |
Date Revised 29.09.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
---|
doi: |
10.1039/c7sc00770a |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM287931757 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM287931757 | ||
003 | DE-627 | ||
005 | 20231225055140.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2017 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1039/c7sc00770a |2 doi | |
028 | 5 | 2 | |a pubmed24n0959.xml |
035 | |a (DE-627)NLM287931757 | ||
035 | |a (NLM)30155213 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Gong, Y |e verfasserin |4 aut | |
245 | 1 | 0 | |a Releasable and traceless PEGylation of arginine-rich antimicrobial peptides |
264 | 1 | |c 2017 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Revised 29.09.2020 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a Arginine-rich antimicrobial peptides (AMPs) are emerging therapeutics of interest. However, their applicability is limited by their short circulation half-life, caused in part by their small size and digestion by blood proteases. This study reports a strategy to temporarily mask arginine residues within AMPs with methoxy poly(ethylene glycol). Based on the reagent used, release of AMPs occurred in hours to days in a completely traceless fashion. In vitro, conjugates were insensitive to serum proteases, and released native AMP with full in vitro bioactivity. This strategy is thus highly relevant and should be adaptable to the entire family of arginine-rich AMPs. It may potentially be used to improve AMP-therapies by providing a more steady concentration of AMP in the blood after a single injection, avoiding toxic effects at high AMP doses, and reducing the number of doses required over the treatment duration | ||
650 | 4 | |a Journal Article | |
700 | 1 | |a Andina, D |e verfasserin |4 aut | |
700 | 1 | |a Nahar, S |e verfasserin |4 aut | |
700 | 1 | |a Leroux, J-C |e verfasserin |4 aut | |
700 | 1 | |a Gauthier, M A |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Chemical science |d 2010 |g 8(2017), 5 vom: 01. Mai, Seite 4082-4086 |w (DE-627)NLM204906237 |x 2041-6520 |7 nnns |
773 | 1 | 8 | |g volume:8 |g year:2017 |g number:5 |g day:01 |g month:05 |g pages:4082-4086 |
856 | 4 | 0 | |u http://dx.doi.org/10.1039/c7sc00770a |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 8 |j 2017 |e 5 |b 01 |c 05 |h 4082-4086 |