Effects of cysteinyl leukotriene receptors on phagocytosis of mouse microglial cells
OBJECTIVE: : To determine the effects of cysteinyl leukotriene receptors (CysLT1R and CysLT2R) on phagocytosis of mouse BV2 microglial cells.
METHODS: : BV2 cells were stimulated with microglial activators lipopolysaccharide (LPS) or CysLT receptor agonists LTD4. The phagocytosis of BV2 cells was observed by immunofluorescence analysis and flow cytometry. The intracellular distributions of CysLT1R and CysLT2R in BV2 cells were examined with immunofluorescence staining.
RESULTS: : Both LPS and LTD4 could significantly enhance the phagocytosis of BV2 cells, and such effect could be inhibited by CysLT1R selective antagonist Montelukast and CysLT2R selective antagonist HAMI 3379. The activation of BV2 cells induced by LTD4 or LPS resulted in changes in intracellular distributions of CysLT1R and CysLT2R. CysLT1R and CysLT2R was co-localization with a similar distribution.
CONCLUSIONS: : CysLT1R and CysLT2R regulate the phagocytosis of mouse BV2 microglial cells with a synergistic effect.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:47 |
|---|---|
| Enthalten in: |
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 47(2018), 1 vom: 25. Jan., Seite 10-18 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Wang, Xiaorong [VerfasserIn] |
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| Anmerkungen: |
Date Completed 09.05.2019 Date Revised 04.08.2023 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM287848957 |
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| 041 | |a chi | ||
| 100 | 1 | |a Wang, Xiaorong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Effects of cysteinyl leukotriene receptors on phagocytosis of mouse microglial cells |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 09.05.2019 | ||
| 500 | |a Date Revised 04.08.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: : To determine the effects of cysteinyl leukotriene receptors (CysLT1R and CysLT2R) on phagocytosis of mouse BV2 microglial cells | ||
| 520 | |a METHODS: : BV2 cells were stimulated with microglial activators lipopolysaccharide (LPS) or CysLT receptor agonists LTD4. The phagocytosis of BV2 cells was observed by immunofluorescence analysis and flow cytometry. The intracellular distributions of CysLT1R and CysLT2R in BV2 cells were examined with immunofluorescence staining | ||
| 520 | |a RESULTS: : Both LPS and LTD4 could significantly enhance the phagocytosis of BV2 cells, and such effect could be inhibited by CysLT1R selective antagonist Montelukast and CysLT2R selective antagonist HAMI 3379. The activation of BV2 cells induced by LTD4 or LPS resulted in changes in intracellular distributions of CysLT1R and CysLT2R. CysLT1R and CysLT2R was co-localization with a similar distribution | ||
| 520 | |a CONCLUSIONS: : CysLT1R and CysLT2R regulate the phagocytosis of mouse BV2 microglial cells with a synergistic effect | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a 3-(((3-carboxycyclohexyl)amino)carbonyl)-4-(3-(4-(4-(cyclohexyloxy)butoxy)phenyl)propoxy)benzoic acid |2 NLM | |
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| 650 | 7 | |a Cyclohexanecarboxylic Acids |2 NLM | |
| 650 | 7 | |a Cyclopropanes |2 NLM | |
| 650 | 7 | |a Lipopolysaccharides |2 NLM | |
| 650 | 7 | |a Phthalic Acids |2 NLM | |
| 650 | 7 | |a Quinolines |2 NLM | |
| 650 | 7 | |a Receptors, Leukotriene |2 NLM | |
| 650 | 7 | |a Sulfides |2 NLM | |
| 650 | 7 | |a cysteinyl leukotriene receptor 2 |2 NLM | |
| 650 | 7 | |a AJT72OTM42 |2 NLM | |
| 650 | 7 | |a leukotriene D4 receptor |2 NLM | |
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| 700 | 1 | |a Lu, Yunbi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Weiping |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Erqing |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Sanhua |e verfasserin |4 aut | |
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