Interferon-γ Induced by Toxoplasma gondii Excreted/Secreted Antigens Promotes Apoptosis of CD4+CD25+ Regulatory T Cells
Objective: To investigate the effect of excreted/secreted antigens (ESAs) from Toxoplasma gondii RH strain and TgCtwh3 strain on apoptosis of CD4+CD25+ regulatory T cells and interferon-γ (IFN-γ) secretion.
Methods: ESAs of Toxoplasma gondii RH strain and TgCtwh3 strain were prepared. Splenic mononuclear cells were isolated from C57BL/6 mice and randomly divided into RH ESA group(2×106 cells/well with addition of 10 μg/ml RH ESA), TgCtwh3 ESA group (2×106 cells/well with addition of 10 μg/ml TgCtwh3 ESA) and control group(2×106 cells/well with addition of 10 μg/ml ovalbumin). Flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells after treatment for 48 h and 72 h. ELISA was conducted to determine the level of IFN-γ in the supernatant after treatment for 72 h. In another experiment, the 3 groups of splenic mononuclear cells were added with 10 μg/ml anti-IFN-γ antibody for 72 h and flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells. Meanwhile, splenic mononuclear cells from IFN-γ knockout and wild-type C57BL/6 mice were also divided into the above-described 3 groups, and flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells after treatment for 72 h.
Results: The concentrations of RH ESA and TgCtwh3 ESA were 0.54 mg/ml and 2.14 mg/ml, respectively. Flow cytometry showed that the early apoptosis rate of CD4+CD25+ regulatory T cells in the RH ESA group and the TgCtwh3 ESA group after 48 h treatment was (12.90±1.26)% and (9.71±1.04)%, respectively (P<0.05), both significantly higher than that in control group (4.48±0.48)% (P<0.01) . The early apoptosis rate of CD4+CD25+ regulatory T cells after 72 h in the RH ESA group was(15.21±1.11)%, significantly higher than that in the TgCtwh3 ESA group[(11.02±0.92)%] (P<0.05) and the control group[(10.10±1.49)%](P<0.01). ELISA showed that the level of interferon-γ in the RH ESA group and the TgCtwh3 ESA group after 72 h was(4 764.0±118.7) pg/ml and (3 629.0±33.6) pg/ml, respectively (P<0.01), both significantly higher than that in the control[(679.4±30.6) pg/ml](P<0.01). Flow cytometry revealed lower early apoptosis rate of CD4+CD25+ regulatory T cells in the RH ESA group added with anti-IFN-γ antibody[(10.44±1.44)%] compared with that without the addition of the antibody[(14.96±0.83)](P<0.05). But this difference was not observed for the TgCtwh3 ESA group. Moreover, the RH ESA-induced apoptosis rate of regulatory T cells from IFN-γ knockout mice[(10.64±0.55)%] was significantly lower than that from the wild-type mice [(15.21±1.11)%](P<0.01). But this difference was not found for the TgCtwh3 ESA treatment.
Conclusion: T. gondii RH ESA induces apoptosis of CD4+CD25+ regulatory T cells and IFN-γ secretion, and these effects are stronger than those of TgCtwh3 ESA. The T. gondii ESA-induced IFN-γ stimulates generation of anti-Toxoplasma immunity during acute Toxoplasma infection through mediation of regulatory T cell apoptosis.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2016 |
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Erschienen: |
2016 |
Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
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Enthalten in: |
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 34(2016), 3 vom: 16. Juni, Seite 183-8 |
Sprache: |
Chinesisch |
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Beteiligte Personen: |
Ge, Ke [VerfasserIn] |
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Themen: |
82115-62-6 |
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Anmerkungen: |
Date Completed 10.10.2018 Date Revised 10.10.2018 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM287629368 |
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100 | 1 | |a Ge, Ke |e verfasserin |4 aut | |
245 | 1 | 0 | |a Interferon-γ Induced by Toxoplasma gondii Excreted/Secreted Antigens Promotes Apoptosis of CD4+CD25+ Regulatory T Cells |
264 | 1 | |c 2016 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 10.10.2018 | ||
500 | |a Date Revised 10.10.2018 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Objective: To investigate the effect of excreted/secreted antigens (ESAs) from Toxoplasma gondii RH strain and TgCtwh3 strain on apoptosis of CD4+CD25+ regulatory T cells and interferon-γ (IFN-γ) secretion | ||
520 | |a Methods: ESAs of Toxoplasma gondii RH strain and TgCtwh3 strain were prepared. Splenic mononuclear cells were isolated from C57BL/6 mice and randomly divided into RH ESA group(2×106 cells/well with addition of 10 μg/ml RH ESA), TgCtwh3 ESA group (2×106 cells/well with addition of 10 μg/ml TgCtwh3 ESA) and control group(2×106 cells/well with addition of 10 μg/ml ovalbumin). Flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells after treatment for 48 h and 72 h. ELISA was conducted to determine the level of IFN-γ in the supernatant after treatment for 72 h. In another experiment, the 3 groups of splenic mononuclear cells were added with 10 μg/ml anti-IFN-γ antibody for 72 h and flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells. Meanwhile, splenic mononuclear cells from IFN-γ knockout and wild-type C57BL/6 mice were also divided into the above-described 3 groups, and flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells after treatment for 72 h | ||
520 | |a Results: The concentrations of RH ESA and TgCtwh3 ESA were 0.54 mg/ml and 2.14 mg/ml, respectively. Flow cytometry showed that the early apoptosis rate of CD4+CD25+ regulatory T cells in the RH ESA group and the TgCtwh3 ESA group after 48 h treatment was (12.90±1.26)% and (9.71±1.04)%, respectively (P<0.05), both significantly higher than that in control group (4.48±0.48)% (P<0.01) . The early apoptosis rate of CD4+CD25+ regulatory T cells after 72 h in the RH ESA group was(15.21±1.11)%, significantly higher than that in the TgCtwh3 ESA group[(11.02±0.92)%] (P<0.05) and the control group[(10.10±1.49)%](P<0.01). ELISA showed that the level of interferon-γ in the RH ESA group and the TgCtwh3 ESA group after 72 h was(4 764.0±118.7) pg/ml and (3 629.0±33.6) pg/ml, respectively (P<0.01), both significantly higher than that in the control[(679.4±30.6) pg/ml](P<0.01). Flow cytometry revealed lower early apoptosis rate of CD4+CD25+ regulatory T cells in the RH ESA group added with anti-IFN-γ antibody[(10.44±1.44)%] compared with that without the addition of the antibody[(14.96±0.83)](P<0.05). But this difference was not observed for the TgCtwh3 ESA group. Moreover, the RH ESA-induced apoptosis rate of regulatory T cells from IFN-γ knockout mice[(10.64±0.55)%] was significantly lower than that from the wild-type mice [(15.21±1.11)%](P<0.01). But this difference was not found for the TgCtwh3 ESA treatment | ||
520 | |a Conclusion: T. gondii RH ESA induces apoptosis of CD4+CD25+ regulatory T cells and IFN-γ secretion, and these effects are stronger than those of TgCtwh3 ESA. The T. gondii ESA-induced IFN-γ stimulates generation of anti-Toxoplasma immunity during acute Toxoplasma infection through mediation of regulatory T cell apoptosis | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Interferon-gamma |2 NLM | |
650 | 7 | |a 82115-62-6 |2 NLM | |
700 | 1 | |a Qiu, Xiao-yan |e verfasserin |4 aut | |
700 | 1 | |a Wang, Li-juan |e verfasserin |4 aut | |
700 | 1 | |a Chen, Hui |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Lu-yan |e verfasserin |4 aut | |
700 | 1 | |a Qiu, Jing-fan |e verfasserin |4 aut | |
700 | 1 | |a Wang, Yong |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases |d 1995 |g 34(2016), 3 vom: 16. Juni, Seite 183-8 |w (DE-627)NLM01284277X |x 1000-7423 |7 nnns |
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912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
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