Novel Orthopoxvirus and Lethal Disease in Cat, Italy
We report detection and full-genome characterization of a novel orthopoxvirus (OPXV) responsible for a fatal infection in a cat. The virus induced skin lesions histologically characterized by leukocyte infiltration and eosinophilic cytoplasmic inclusions. Different PCR approaches were unable to assign the virus to a defined OPXV species. Large amounts of typical brick-shaped virions, morphologically related to OPXV, were observed by electron microscopy. This OPXV strain (Italy_09/17) was isolated on cell cultures and embryonated eggs. Phylogenetic analysis of 9 concatenated genes showed that this virus was distantly related to cowpox virus, more closely related to to ectromelia virus, and belonged to the same cluster of an OPXV recently isolated from captive macaques in Italy. Extensive epidemiologic surveillance in cats and rodents will assess whether cats are incidental hosts and rodents are the main reservoir of the virus. The zoonotic potential of this novel virus also deserves further investigation.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2018 |
|---|---|
| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
|---|---|
| Enthalten in: |
Emerging infectious diseases - 24(2018), 9 vom: 20. Sept., Seite 1665-1673 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Lanave, Gianvito [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 22.04.2019 Date Revised 22.04.2019 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.3201/eid2409.171283 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM287628574 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM287628574 | ||
| 003 | DE-627 | ||
| 005 | 20231225054435.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3201/eid2409.171283 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0958.xml |
| 035 | |a (DE-627)NLM287628574 | ||
| 035 | |a (NLM)30124195 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Lanave, Gianvito |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Novel Orthopoxvirus and Lethal Disease in Cat, Italy |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.04.2019 | ||
| 500 | |a Date Revised 22.04.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We report detection and full-genome characterization of a novel orthopoxvirus (OPXV) responsible for a fatal infection in a cat. The virus induced skin lesions histologically characterized by leukocyte infiltration and eosinophilic cytoplasmic inclusions. Different PCR approaches were unable to assign the virus to a defined OPXV species. Large amounts of typical brick-shaped virions, morphologically related to OPXV, were observed by electron microscopy. This OPXV strain (Italy_09/17) was isolated on cell cultures and embryonated eggs. Phylogenetic analysis of 9 concatenated genes showed that this virus was distantly related to cowpox virus, more closely related to to ectromelia virus, and belonged to the same cluster of an OPXV recently isolated from captive macaques in Italy. Extensive epidemiologic surveillance in cats and rodents will assess whether cats are incidental hosts and rodents are the main reservoir of the virus. The zoonotic potential of this novel virus also deserves further investigation | ||
| 650 | 4 | |a Case Reports | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Italy | |
| 650 | 4 | |a bioterrorism and preparedness | |
| 650 | 4 | |a cat | |
| 650 | 4 | |a lethal disease | |
| 650 | 4 | |a next-generation sequencing | |
| 650 | 4 | |a novel orthopoxvirus | |
| 650 | 4 | |a orthopoxvirus | |
| 650 | 4 | |a phylogeny | |
| 650 | 4 | |a viruses | |
| 650 | 4 | |a zoonoses | |
| 700 | 1 | |a Dowgier, Giulia |e verfasserin |4 aut | |
| 700 | 1 | |a Decaro, Nicola |e verfasserin |4 aut | |
| 700 | 1 | |a Albanese, Francesco |e verfasserin |4 aut | |
| 700 | 1 | |a Brogi, Elisa |e verfasserin |4 aut | |
| 700 | 1 | |a Parisi, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Losurdo, Michele |e verfasserin |4 aut | |
| 700 | 1 | |a Lavazza, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Martella, Vito |e verfasserin |4 aut | |
| 700 | 1 | |a Buonavoglia, Canio |e verfasserin |4 aut | |
| 700 | 1 | |a Elia, Gabriella |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Emerging infectious diseases |d 1995 |g 24(2018), 9 vom: 20. Sept., Seite 1665-1673 |w (DE-627)NLM088704254 |x 1080-6059 |7 nnns |
| 773 | 1 | 8 | |g volume:24 |g year:2018 |g number:9 |g day:20 |g month:09 |g pages:1665-1673 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3201/eid2409.171283 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 24 |j 2018 |e 9 |b 20 |c 09 |h 1665-1673 | ||