Rationally designed perturbation factor drives evolution in Saccharomyces cerevisiae for industrial application
Saccharomyces cerevisiae strains with favorable characteristics are preferred for application in industries. However, the current ability to reprogram a yeast cell on the genome scale is limited due to the complexity of yeast ploids. In this study, a method named genome replication engineering-assisted continuous evolution (GREACE) was proved efficient in engineering S. cerevisiae with different ploids. Through iterative cycles of culture coupled with selection, GREACE could continuously improve the target traits of yeast by accumulating beneficial genetic modification in genome. The application of GREACE greatly improved the tolerance of yeast against acetic acid compared with their parent strain. This method could also be employed to improve yeast aroma profile and the phenotype could be stably inherited to the offspring. Therefore, GREACE method was efficient in S. cerevisiae engineering and it could be further used to evolve yeast with other specific characteristics.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:45 |
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| Enthalten in: |
Journal of industrial microbiology & biotechnology - 45(2018), 10 vom: 04. Okt., Seite 869-880 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xu, Xin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 27.11.2018 Date Revised 27.11.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s10295-018-2057-x |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM287161013 |
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| 245 | 1 | 0 | |a Rationally designed perturbation factor drives evolution in Saccharomyces cerevisiae for industrial application |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Saccharomyces cerevisiae strains with favorable characteristics are preferred for application in industries. However, the current ability to reprogram a yeast cell on the genome scale is limited due to the complexity of yeast ploids. In this study, a method named genome replication engineering-assisted continuous evolution (GREACE) was proved efficient in engineering S. cerevisiae with different ploids. Through iterative cycles of culture coupled with selection, GREACE could continuously improve the target traits of yeast by accumulating beneficial genetic modification in genome. The application of GREACE greatly improved the tolerance of yeast against acetic acid compared with their parent strain. This method could also be employed to improve yeast aroma profile and the phenotype could be stably inherited to the offspring. Therefore, GREACE method was efficient in S. cerevisiae engineering and it could be further used to evolve yeast with other specific characteristics | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Acetaldehyde | |
| 650 | 4 | |a Acetic acid tolerance | |
| 650 | 4 | |a DNA polymerase | |
| 650 | 4 | |a GREACE method | |
| 650 | 4 | |a Saccharomyces cerevisiae | |
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| 700 | 1 | |a Liu, Chunfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Chengtuo |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jinjing |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Feiyun |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yongxian |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Qi |e verfasserin |4 aut | |
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