MRP8/14 does not contribute to dissemination or inflammation in a murine model of Lyme borreliosis
Copyright © 2018 The Authors. Published by Elsevier GmbH.. All rights reserved..
Myeloid-related protein (MRP)8 and MRP14 form a complex (MRP8/14) that is released by activated neutrophils and monocytes during infection. MRP8/14 has been shown to have bacteriostatic activity in vitro against Borrelia burgdorferi, the spirochete that causes Lyme borreliosis. Furthermore, levels of MRP8/14 have been shown to be elevated in the joints of patients with Lyme arthritis. We hypothesized that MRP8/14 has a protective effect during B. burgdorferi infection. To determine the role of MRP8/14 in the immune response to B. burgdorferi, we studied the course of B. burgdorferi infection in wildtype (wt) and mrp14-/- mice. In addition, we studied the response of leukocytes from mice lacking MRP8/14 to B. burgdorferi ex vivo. We demonstrated similar levels of B. burgdorferi dissemination, cytokine and immunoglobulin production in infected wt and mrp14-/- mice after 21 days. Neutrophils and monocytes lacking MRP8/14 were undiminished in their ability to become activated or phagocytose B. burgdorferi. In conclusion, we did not find a central role of MRP8/14 in the immune response against B. burgdorferi. As the levels of MRP8/14 in the serum of infected mice were low, we speculate that MRP8/14 is not released in levels great enough to influence the course of B. burgdorferi infection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:223 |
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| Enthalten in: |
Immunobiology - 223(2018), 11 vom: 15. Nov., Seite 694-698 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mason, Lauren M K [VerfasserIn] |
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| Links: |
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| Themen: |
Borrelia |
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| Anmerkungen: |
Date Completed 25.06.2019 Date Revised 25.06.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.imbio.2018.07.017 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM286969203 |
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| 100 | 1 | |a Mason, Lauren M K |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a MRP8/14 does not contribute to dissemination or inflammation in a murine model of Lyme borreliosis |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 The Authors. Published by Elsevier GmbH.. All rights reserved. | ||
| 520 | |a Myeloid-related protein (MRP)8 and MRP14 form a complex (MRP8/14) that is released by activated neutrophils and monocytes during infection. MRP8/14 has been shown to have bacteriostatic activity in vitro against Borrelia burgdorferi, the spirochete that causes Lyme borreliosis. Furthermore, levels of MRP8/14 have been shown to be elevated in the joints of patients with Lyme arthritis. We hypothesized that MRP8/14 has a protective effect during B. burgdorferi infection. To determine the role of MRP8/14 in the immune response to B. burgdorferi, we studied the course of B. burgdorferi infection in wildtype (wt) and mrp14-/- mice. In addition, we studied the response of leukocytes from mice lacking MRP8/14 to B. burgdorferi ex vivo. We demonstrated similar levels of B. burgdorferi dissemination, cytokine and immunoglobulin production in infected wt and mrp14-/- mice after 21 days. Neutrophils and monocytes lacking MRP8/14 were undiminished in their ability to become activated or phagocytose B. burgdorferi. In conclusion, we did not find a central role of MRP8/14 in the immune response against B. burgdorferi. As the levels of MRP8/14 in the serum of infected mice were low, we speculate that MRP8/14 is not released in levels great enough to influence the course of B. burgdorferi infection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Borrelia | |
| 650 | 4 | |a Calprotectin | |
| 650 | 4 | |a Lyme | |
| 650 | 4 | |a MRP8/14 | |
| 650 | 4 | |a S100A8 | |
| 650 | 4 | |a S100A9 | |
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| 700 | 1 | |a Coumou, Jeroen |e verfasserin |4 aut | |
| 700 | 1 | |a Ersöz, Jasmin I |e verfasserin |4 aut | |
| 700 | 1 | |a Oei, Anneke |e verfasserin |4 aut | |
| 700 | 1 | |a Roelofs, Joris J T H |e verfasserin |4 aut | |
| 700 | 1 | |a Vogl, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a van der Poll, Tom |e verfasserin |4 aut | |
| 700 | 1 | |a Hovius, Joppe W R |e verfasserin |4 aut | |
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