Dynamic changes of DNA methylation and lung disease in cystic fibrosis : lessons from a monogenic disease
AIM: To assess whether DNA methylation levels account for the noninherited phenotypic variations observed among cystic fibrosis (CF) patients.
PATIENTS & METHODS: Using the 450 K BeadChip, we profiled DNA methylation in nasal epithelial cells collected from 32 CF patients and 16 controls.
RESULTS: We detected substantial DNA methylation differences up to 55% (median β change 0.13; IQR: 0.15-0.11) between CF patients and controls. DNA methylation levels differed between mild and severe CF patients and correlated with lung function at 50 CpG sites.
CONCLUSION: In CF samples, dynamic changes of DNA methylation occurred in genes responsible for the integrity of the epithelium and the inflammatory and immune responses, were prominent in transcriptionally active genomic regions and were over-represented in enhancers active in lung tissues. ( Clinicaltrials.gov NCT02884622).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
Epigenomics - 10(2018), 8 vom: 01. Aug., Seite 1131-1145 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Magalhães, Milena [VerfasserIn] |
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| Links: |
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| Themen: |
Cystic fibrosis |
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| Anmerkungen: |
Date Completed 23.05.2019 Date Revised 23.05.2019 published: Print-Electronic ClinicalTrials.gov: NCT02884622 Citation Status MEDLINE |
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| doi: |
10.2217/epi-2018-0005 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM286920778 |
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| 500 | |a Date Revised 23.05.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a ClinicalTrials.gov: NCT02884622 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a AIM: To assess whether DNA methylation levels account for the noninherited phenotypic variations observed among cystic fibrosis (CF) patients | ||
| 520 | |a PATIENTS & METHODS: Using the 450 K BeadChip, we profiled DNA methylation in nasal epithelial cells collected from 32 CF patients and 16 controls | ||
| 520 | |a RESULTS: We detected substantial DNA methylation differences up to 55% (median β change 0.13; IQR: 0.15-0.11) between CF patients and controls. DNA methylation levels differed between mild and severe CF patients and correlated with lung function at 50 CpG sites | ||
| 520 | |a CONCLUSION: In CF samples, dynamic changes of DNA methylation occurred in genes responsible for the integrity of the epithelium and the inflammatory and immune responses, were prominent in transcriptionally active genomic regions and were over-represented in enhancers active in lung tissues. ( Clinicaltrials.gov NCT02884622) | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a DNA methylation | |
| 650 | 4 | |a cystic fibrosis | |
| 650 | 4 | |a epigenetics and disease | |
| 700 | 1 | |a Tost, Jörg |e verfasserin |4 aut | |
| 700 | 1 | |a Pineau, Fanny |e verfasserin |4 aut | |
| 700 | 1 | |a Rivals, Isabelle |e verfasserin |4 aut | |
| 700 | 1 | |a Busato, Florence |e verfasserin |4 aut | |
| 700 | 1 | |a Alary, Nathan |e verfasserin |4 aut | |
| 700 | 1 | |a Mely, Laurent |e verfasserin |4 aut | |
| 700 | 1 | |a Leroy, Sylvie |e verfasserin |4 aut | |
| 700 | 1 | |a Murris, Marlène |e verfasserin |4 aut | |
| 700 | 1 | |a Caimmi, Davide |e verfasserin |4 aut | |
| 700 | 1 | |a Claustres, Mireille |e verfasserin |4 aut | |
| 700 | 1 | |a Chiron, Raphaël |e verfasserin |4 aut | |
| 700 | 1 | |a De Sario, Albertina |e verfasserin |4 aut | |
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