Evaluation of some potential target genes and methods for RNAi-mediated pest control of the corn earworm Helicoverpa zea
Copyright © 2018. Published by Elsevier Inc..
In this study, we explored the efficacy of knockdown four genes required for proper nervous system function by RNAi, in the corn earworm Helicoverpa zea (Boddie). Three of these genes encode components of validated insecticide target sites. We synthesized cDNA sequences orthologous to the Drosophila melanogaster genes Para (paralyticts), TipE (temperature-induced paralysis locus E), GluCl (glutamate-gated chloride channel), and Notch, and used these fragments to synthesize double-stranded RNAs (dsRNAs). We then performed experiments in an attempt to induce RNAi-mediated effects on gene expression and viability using three modes of delivery of the dsRNAs: microinjection of eggs, soaking of eggs and feeding of larvae. Microinjection of dsRNAs into eggs induced reduced hatch rates and knockdown of target gene expression for GluCl, para and TipE, but not for Notch. However, neither feeding nor soaking eggs in dsRNA solutions resulted in discernable RNAi effects. These results demonstrated the susceptibility to RNAi effects of the expression of H. zea genes encoding insecticide target sites, which suggests future avenues of research toward practical applications.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:149 |
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| Enthalten in: |
Pesticide biochemistry and physiology - 149(2018) vom: 01. Juli, Seite 67-72 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Jinda [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.08.2018 Date Revised 13.06.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.pestbp.2018.05.012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Date Revised 13.06.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018. Published by Elsevier Inc. | ||
| 520 | |a In this study, we explored the efficacy of knockdown four genes required for proper nervous system function by RNAi, in the corn earworm Helicoverpa zea (Boddie). Three of these genes encode components of validated insecticide target sites. We synthesized cDNA sequences orthologous to the Drosophila melanogaster genes Para (paralyticts), TipE (temperature-induced paralysis locus E), GluCl (glutamate-gated chloride channel), and Notch, and used these fragments to synthesize double-stranded RNAs (dsRNAs). We then performed experiments in an attempt to induce RNAi-mediated effects on gene expression and viability using three modes of delivery of the dsRNAs: microinjection of eggs, soaking of eggs and feeding of larvae. Microinjection of dsRNAs into eggs induced reduced hatch rates and knockdown of target gene expression for GluCl, para and TipE, but not for Notch. However, neither feeding nor soaking eggs in dsRNA solutions resulted in discernable RNAi effects. These results demonstrated the susceptibility to RNAi effects of the expression of H. zea genes encoding insecticide target sites, which suggests future avenues of research toward practical applications | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Corn earworm (Helicoverpa zea) | |
| 650 | 4 | |a Insecticide target | |
| 650 | 4 | |a RNA interference (RNAi) | |
| 650 | 4 | |a dsRNA delivery | |
| 650 | 7 | |a Chloride Channels |2 NLM | |
| 650 | 7 | |a Drosophila Proteins |2 NLM | |
| 650 | 7 | |a Insecticides |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
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| 650 | 7 | |a Receptors, Notch |2 NLM | |
| 650 | 7 | |a TipE protein, Drosophila |2 NLM | |
| 650 | 7 | |a glutamate-gated chloride channels |2 NLM | |
| 700 | 1 | |a Gu, Liuqi |e verfasserin |4 aut | |
| 700 | 1 | |a Knipple, Douglas C |e verfasserin |4 aut | |
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