The use of chemical probes to detect the proteomics of renal tubular injury induced by maleic acid
Copyright © 2018 Elsevier B.V. All rights reserved..
Maleic acid (MA), an industrial raw material, was found to be illegally added to edible starch-based food products in Taiwan in 2013, a practice unheard of in most of the world. MA has been associated with renal dysfunction in many experimental animal studies. In this study, we developed chemical probes to investigate protein-protein interactions between MA and renal proteins. In the fabrication of the MA probes, we used silicon dioxide (SiO2) modified with a silanized linker (3-aminopropyl triethoxyslane, APTES) to generate MA with APTES-SiO2 particles. The probes were then incubated with the cell lysates of normal human kidney cell lines (HK-2) and subjected to MS/MS for identifying several MA-related proteins, including nucleophosmin, neutral alpha-glucosidase AB, translocon-associated protein subunit alpha, elongation factor 1-gamma, 60S acidic ribosomal protein P0-like, and heat shock protein (HSP 90-alpha and beta). Based on our findings, we believed that the probe can potentially be used to identify and detect the target proteins and help characterize a network of MA protein-protein interactions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1565 |
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| Enthalten in: |
Journal of chromatography. A - 1565(2018) vom: 31. Aug., Seite 96-104 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lin, Hugo You-Hsien [VerfasserIn] |
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| Links: |
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| Themen: |
7631-86-9 |
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| Anmerkungen: |
Date Completed 13.08.2018 Date Revised 02.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.chroma.2018.06.040 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM286258897 |
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| 245 | 1 | 4 | |a The use of chemical probes to detect the proteomics of renal tubular injury induced by maleic acid |
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| 500 | |a Date Completed 13.08.2018 | ||
| 500 | |a Date Revised 02.12.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 Elsevier B.V. All rights reserved. | ||
| 520 | |a Maleic acid (MA), an industrial raw material, was found to be illegally added to edible starch-based food products in Taiwan in 2013, a practice unheard of in most of the world. MA has been associated with renal dysfunction in many experimental animal studies. In this study, we developed chemical probes to investigate protein-protein interactions between MA and renal proteins. In the fabrication of the MA probes, we used silicon dioxide (SiO2) modified with a silanized linker (3-aminopropyl triethoxyslane, APTES) to generate MA with APTES-SiO2 particles. The probes were then incubated with the cell lysates of normal human kidney cell lines (HK-2) and subjected to MS/MS for identifying several MA-related proteins, including nucleophosmin, neutral alpha-glucosidase AB, translocon-associated protein subunit alpha, elongation factor 1-gamma, 60S acidic ribosomal protein P0-like, and heat shock protein (HSP 90-alpha and beta). Based on our findings, we believed that the probe can potentially be used to identify and detect the target proteins and help characterize a network of MA protein-protein interactions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chemical probes | |
| 650 | 4 | |a Human kidney cell lines (HK-2) | |
| 650 | 4 | |a Maleic acid (MA) | |
| 650 | 4 | |a Protein–protein interactions | |
| 650 | 4 | |a Proteomics | |
| 650 | 7 | |a Maleates |2 NLM | |
| 650 | 7 | |a Molecular Probes |2 NLM | |
| 650 | 7 | |a Proteins |2 NLM | |
| 650 | 7 | |a Silicon Dioxide |2 NLM | |
| 650 | 7 | |a 7631-86-9 |2 NLM | |
| 650 | 7 | |a maleic acid |2 NLM | |
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| 700 | 1 | |a Liang, Chan-Jung |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Meng-Chieh |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Mei-Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Jung-San |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Shih-Shin |e verfasserin |4 aut | |
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