A Rapid and Specific Assay for the Detection of MERS-CoV
Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel human coronavirus that can cause human respiratory disease. The development of a detection method for this virus that can lead to rapid and accurate diagnosis would be significant. In this study, we established a nucleic acid visualization technique that combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualization strip (RT-LAMP-VF) to detect the N gene of MERS-CoV. The RT-LAMP-VF assay was performed in a constant temperature water bath for 30 min, and the result was visible by the naked eye within 5 min. The RT-LAMP-VF assay was capable of detecting 2 × 101 copies/μl of synthesized RNA transcript and 1 × 101 copies/μl of MERS-CoV RNA. The method exhibits no cross-reactivities with multiple CoVs including SARS-related (SARSr)-CoV, HKU4, HKU1, OC43 and 229E, and thus exhibits high specificity. Compared to the real-time RT-PCR (rRT-PCR) method recommended by the World Health Organization (WHO), the RT-LAMP-VF assay is easy to handle, does not require expensive equipment and can rapidly complete detection within 35 min.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
Frontiers in microbiology - 9(2018) vom: 01., Seite 1101 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Pei [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Revised 30.09.2020 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3389/fmicb.2018.01101 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM285405799 |
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| 520 | |a Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel human coronavirus that can cause human respiratory disease. The development of a detection method for this virus that can lead to rapid and accurate diagnosis would be significant. In this study, we established a nucleic acid visualization technique that combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualization strip (RT-LAMP-VF) to detect the N gene of MERS-CoV. The RT-LAMP-VF assay was performed in a constant temperature water bath for 30 min, and the result was visible by the naked eye within 5 min. The RT-LAMP-VF assay was capable of detecting 2 × 101 copies/μl of synthesized RNA transcript and 1 × 101 copies/μl of MERS-CoV RNA. The method exhibits no cross-reactivities with multiple CoVs including SARS-related (SARSr)-CoV, HKU4, HKU1, OC43 and 229E, and thus exhibits high specificity. Compared to the real-time RT-PCR (rRT-PCR) method recommended by the World Health Organization (WHO), the RT-LAMP-VF assay is easy to handle, does not require expensive equipment and can rapidly complete detection within 35 min | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Middle East respiratory syndrome coronavirus | |
| 650 | 4 | |a RT-LAMP-VF | |
| 650 | 4 | |a nucleic acid visualization | |
| 650 | 4 | |a reverse transcription loop-mediated isothermal amplification | |
| 650 | 4 | |a visual detection | |
| 700 | 1 | |a Wang, Hualei |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Zengguo |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Hongli |e verfasserin |4 aut | |
| 700 | 1 | |a Chi, Hang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Jincun |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Beibei |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Feihu |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Xingxing |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Fangfang |e verfasserin |4 aut | |
| 700 | 1 | |a Jiao, Cuicui |e verfasserin |4 aut | |
| 700 | 1 | |a Hou, Pengfei |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Shengnan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Yongkun |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jianzhong |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Weiyang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Tiecheng |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Yuwei |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Songtao |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Xianzhu |e verfasserin |4 aut | |
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