Lithium chloride arrests HK-2 cell cycle in G2 phase through AKT/GSK-3β signal pathway
OBJECTIVE: To investigate the effect of lithium chloride (LiCl) on cell cycle of HK-2 cells and explore the possible pathways involved.
METHODS: HK-2 cells were treated with LiCl at different concentrations (5, 12.5, 20, and 25 mmol/L) for 12, 24, 48, or 72 h, and the changes in cell cycle and viability were detected using flow cytometry and CCK-8 assay, respectively. Western blotting was used to analyze the changes in the expressions of cyclin B1 and CDK1 (the two G2 phase-related proteins) and those of AKT/GSK-3β signaling pathway-related proteins in the treated cells.
RESULTS: LiCl treatment time- and concentration-dependently increased HK-2 cell percentage in G2 phase and decreased the cell vitality. The expressions of cyclin B1, CDK1, p-GSK-3β, and β-catenin increased and the expression of p-AKT decreased significantly in the cells as LiCl treatment time and concentration increased.
CONCLUSION: LiCl may cause HK-2 cell cycle arrest in G2 phase through activation of the AKT/GSK-3β signaling pathway.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:38 |
|---|---|
| Enthalten in: |
Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 38(2018), 5 vom: 20. Mai, Seite 541-546 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Jiang, Ting-Ting [VerfasserIn] |
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| Themen: |
Beta Catenin |
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| Anmerkungen: |
Date Completed 04.02.2019 Date Revised 07.09.2023 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM285359282 |
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| LEADER | 01000naa a22002652 4500 | ||
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| 003 | DE-627 | ||
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| 100 | 1 | |a Jiang, Ting-Ting |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Lithium chloride arrests HK-2 cell cycle in G2 phase through AKT/GSK-3β signal pathway |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 04.02.2019 | ||
| 500 | |a Date Revised 07.09.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To investigate the effect of lithium chloride (LiCl) on cell cycle of HK-2 cells and explore the possible pathways involved | ||
| 520 | |a METHODS: HK-2 cells were treated with LiCl at different concentrations (5, 12.5, 20, and 25 mmol/L) for 12, 24, 48, or 72 h, and the changes in cell cycle and viability were detected using flow cytometry and CCK-8 assay, respectively. Western blotting was used to analyze the changes in the expressions of cyclin B1 and CDK1 (the two G2 phase-related proteins) and those of AKT/GSK-3β signaling pathway-related proteins in the treated cells | ||
| 520 | |a RESULTS: LiCl treatment time- and concentration-dependently increased HK-2 cell percentage in G2 phase and decreased the cell vitality. The expressions of cyclin B1, CDK1, p-GSK-3β, and β-catenin increased and the expression of p-AKT decreased significantly in the cells as LiCl treatment time and concentration increased | ||
| 520 | |a CONCLUSION: LiCl may cause HK-2 cell cycle arrest in G2 phase through activation of the AKT/GSK-3β signaling pathway | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Cyclin B1 |2 NLM | |
| 650 | 7 | |a beta Catenin |2 NLM | |
| 650 | 7 | |a Glycogen Synthase Kinase 3 beta |2 NLM | |
| 650 | 7 | |a EC 2.7.11.1 |2 NLM | |
| 650 | 7 | |a Proto-Oncogene Proteins c-akt |2 NLM | |
| 650 | 7 | |a EC 2.7.11.1 |2 NLM | |
| 650 | 7 | |a CDC2 Protein Kinase |2 NLM | |
| 650 | 7 | |a EC 2.7.11.22 |2 NLM | |
| 650 | 7 | |a CDK1 protein, human |2 NLM | |
| 650 | 7 | |a EC 2.7.11.22 |2 NLM | |
| 650 | 7 | |a Lithium Chloride |2 NLM | |
| 650 | 7 | |a G4962QA067 |2 NLM | |
| 700 | 1 | |a Zhang, Wen-Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Xiang, Xiao-Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Shu, Shuang-Shuang |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Xun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jun |e verfasserin |4 aut | |
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