Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2
Copyright © 2018. Published by Elsevier Ltd..
An O-specific polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O50 followed by gel chromatography on Sephadex G-50. The following structure of the tetrasaccharide repeat was established by sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy: →3)-α-l-Rhap-(1 → 2)-α-l-Rhap-(1 → 3)-β-l-Rhap-(1 → 4)-β-d-GlcpNAc-(1→ The linear O50 polysaccharide has the same structure as the main chain of the branched O polysaccharide of E. coli O2 studied earlier [Jansson et al., Carbohydr. Res. 161 (1987) 273-279], which differs in the presence of a side-chain α-d-Fucp3NAc residue. In spite of the difference between the O-polysaccharides, the corresponding genes in the O2- and O50-antigen gene cluster are 99-100% identical. The genetic basis for the lack of d-Fucp3NAc from the O50 polysaccharide is evidently a point mutation in the aminotransferase gene fdtB of the d-Fucp3NAc synthesis pathway resulting in a single amino acid change from histidine in O2 to arginine in O50.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:464 |
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| Enthalten in: |
Carbohydrate research - 464(2018) vom: 15. Juli, Seite 8-11 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, Bin [VerfasserIn] |
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| Links: |
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| Themen: |
Bacterial polysaccharide structure |
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| Anmerkungen: |
Date Completed 01.10.2018 Date Revised 01.10.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.carres.2018.05.001 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM284337382 |
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| 500 | |a Date Revised 01.10.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018. Published by Elsevier Ltd. | ||
| 520 | |a An O-specific polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O50 followed by gel chromatography on Sephadex G-50. The following structure of the tetrasaccharide repeat was established by sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy: →3)-α-l-Rhap-(1 → 2)-α-l-Rhap-(1 → 3)-β-l-Rhap-(1 → 4)-β-d-GlcpNAc-(1→ The linear O50 polysaccharide has the same structure as the main chain of the branched O polysaccharide of E. coli O2 studied earlier [Jansson et al., Carbohydr. Res. 161 (1987) 273-279], which differs in the presence of a side-chain α-d-Fucp3NAc residue. In spite of the difference between the O-polysaccharides, the corresponding genes in the O2- and O50-antigen gene cluster are 99-100% identical. The genetic basis for the lack of d-Fucp3NAc from the O50 polysaccharide is evidently a point mutation in the aminotransferase gene fdtB of the d-Fucp3NAc synthesis pathway resulting in a single amino acid change from histidine in O2 to arginine in O50 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bacterial polysaccharide structure | |
| 650 | 4 | |a Escherichia coli | |
| 650 | 4 | |a Lipopolysaccharide | |
| 650 | 4 | |a O-antigen | |
| 650 | 4 | |a O-antigen gene cluster | |
| 650 | 4 | |a O-specific polysaccharide | |
| 650 | 7 | |a O Antigens |2 NLM | |
| 700 | 1 | |a Senchenkova, Sof'ya N |e verfasserin |4 aut | |
| 700 | 1 | |a Naumenko, Olesya I |e verfasserin |4 aut | |
| 700 | 1 | |a Shashkov, Alexander S |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Perepelov, Andrey V |e verfasserin |4 aut | |
| 700 | 1 | |a Knirel, Yuriy A |e verfasserin |4 aut | |
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