Solitary chemosensory cells are a primary epithelial source of IL-25 in patients with chronic rhinosinusitis with nasal polyps
Published by Elsevier Inc..
BACKGROUND: IL-25 can function as an early signal for the respiratory type 2 response characteristic of allergic asthma and chronic rhinosinusitis with nasal polyps (CRSwNP). In the mouse gut, tuft cells are the epithelial source of IL-25. However, the source of human airway epithelial IL-25 has remained elusive.
OBJECTIVE: In this study we sought to determine whether the solitary chemosensory cell (SCC) is the predominant source of IL-25 in the sinonasal epithelium.
METHOD: Flow cytometry and immunofluorescence for SCCs and IL-25 were used to interrogate polyp and turbinate tissue from patients with CRSwNP. Mucus was collected during acute inflammatory exacerbations from patients with CRSwNP or chronic rhinosinusitis without nasal polyps and IL-25 levels determined by using ELISA. Lastly, sinonasal epithelial cultures derived from polyp and turbinate tissue were stimulated with IL-13 and analyzed for SCC proliferation and IL-25 production.
RESULTS: This study demonstrates that a discrete cell type, likely an SCC, characterized by expression of the taste-associated G protein gustducin and the intestinal tuft cell marker doublecortin-like kinase 1, is the predominant source of IL-25 in the human upper airway. Additionally, we show that patients with CRSwNP have increased numbers of SCCs in nasal polyp tissue and that in vitro IL-13 exposure both increased proliferation and induced apical secretion of IL-25 into the mucosal layer.
CONCLUSIONS: Inflammatory sinus polyps but not adjacent turbinate tissue show expansion of the SCC population, which is the source of epithelial IL-25.
| Errataetall: |
CommentIn: J Allergy Clin Immunol. 2018 Aug;142(2):403-404. - PMID 29928925 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:142 |
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| Enthalten in: |
The Journal of allergy and clinical immunology - 142(2018), 2 vom: 02. Aug., Seite 460-469.e7 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kohanski, Michael A [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 19.08.2019 Date Revised 16.09.2023 published: Print-Electronic CommentIn: J Allergy Clin Immunol. 2018 Aug;142(2):403-404. - PMID 29928925 Citation Status MEDLINE |
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| doi: |
10.1016/j.jaci.2018.03.019 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 100 | 1 | |a Kohanski, Michael A |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Solitary chemosensory cells are a primary epithelial source of IL-25 in patients with chronic rhinosinusitis with nasal polyps |
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| 500 | |a Date Completed 19.08.2019 | ||
| 500 | |a Date Revised 16.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a CommentIn: J Allergy Clin Immunol. 2018 Aug;142(2):403-404. - PMID 29928925 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Published by Elsevier Inc. | ||
| 520 | |a BACKGROUND: IL-25 can function as an early signal for the respiratory type 2 response characteristic of allergic asthma and chronic rhinosinusitis with nasal polyps (CRSwNP). In the mouse gut, tuft cells are the epithelial source of IL-25. However, the source of human airway epithelial IL-25 has remained elusive | ||
| 520 | |a OBJECTIVE: In this study we sought to determine whether the solitary chemosensory cell (SCC) is the predominant source of IL-25 in the sinonasal epithelium | ||
| 520 | |a METHOD: Flow cytometry and immunofluorescence for SCCs and IL-25 were used to interrogate polyp and turbinate tissue from patients with CRSwNP. Mucus was collected during acute inflammatory exacerbations from patients with CRSwNP or chronic rhinosinusitis without nasal polyps and IL-25 levels determined by using ELISA. Lastly, sinonasal epithelial cultures derived from polyp and turbinate tissue were stimulated with IL-13 and analyzed for SCC proliferation and IL-25 production | ||
| 520 | |a RESULTS: This study demonstrates that a discrete cell type, likely an SCC, characterized by expression of the taste-associated G protein gustducin and the intestinal tuft cell marker doublecortin-like kinase 1, is the predominant source of IL-25 in the human upper airway. Additionally, we show that patients with CRSwNP have increased numbers of SCCs in nasal polyp tissue and that in vitro IL-13 exposure both increased proliferation and induced apical secretion of IL-25 into the mucosal layer | ||
| 520 | |a CONCLUSIONS: Inflammatory sinus polyps but not adjacent turbinate tissue show expansion of the SCC population, which is the source of epithelial IL-25 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a IL-13 | |
| 650 | 4 | |a IL-25 | |
| 650 | 4 | |a chronic rhinosinusitis with nasal polyps | |
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| 650 | 4 | |a solitary chemosensory cells | |
| 650 | 4 | |a type 2 inflammation | |
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| 650 | 7 | |a Interleukin-17 |2 NLM | |
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| 700 | 1 | |a Shtraks, Julie P |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Bei |e verfasserin |4 aut | |
| 700 | 1 | |a Blasetti, Mariel |e verfasserin |4 aut | |
| 700 | 1 | |a Doghramji, Laurel |e verfasserin |4 aut | |
| 700 | 1 | |a Kennedy, David W |e verfasserin |4 aut | |
| 700 | 1 | |a Adappa, Nithin D |e verfasserin |4 aut | |
| 700 | 1 | |a Palmer, James N |e verfasserin |4 aut | |
| 700 | 1 | |a Herbert, De'Broski R |e verfasserin |4 aut | |
| 700 | 1 | |a Cohen, Noam A |e verfasserin |4 aut | |
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