Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection
Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved..
BACKGROUND: Information on the duration of replicative Middle East respiratory syndrome coronavirus (MERS-CoV) shedding is important for infection control. The detection of MERS-CoV sub-genomic mRNAs indicates that the virus is replicative. This study examined the duration for detecting MERS-CoV sub-genomic mRNA compared with genomic RNA in diverse respiratory specimens.
METHODS: Upper and lower respiratory samples were obtained from 17 MERS-CoV-infected patients. MERS-CoV sub-genomic mRNA was detected by reverse transcription PCR (RT-PCR) and MERS-CoV genomic RNA by real-time RT-PCR.
RESULTS: In sputum and transtracheal aspirate, sub-genomic mRNA was detected for up to 4 weeks after symptoms developed, which correlated with the detection of genomic RNA. In oropharyngeal and nasopharyngeal swab specimens, the detection of sub-genomic mRNA and genomic RNA did not correlate.
CONCLUSIONS: These findings suggest that MERS-CoV does not replicate well in the upper respiratory tract.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:72 |
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| Enthalten in: |
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 72(2018) vom: 15. Juli, Seite 8-10 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Park, Wan Beom [VerfasserIn] |
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| Links: |
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| Themen: |
Coronavirus infections |
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| Anmerkungen: |
Date Completed 13.11.2018 Date Revised 07.04.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijid.2018.05.003 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM283998326 |
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| 245 | 1 | 0 | |a Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection |
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| 500 | |a Date Completed 13.11.2018 | ||
| 500 | |a Date Revised 07.04.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a BACKGROUND: Information on the duration of replicative Middle East respiratory syndrome coronavirus (MERS-CoV) shedding is important for infection control. The detection of MERS-CoV sub-genomic mRNAs indicates that the virus is replicative. This study examined the duration for detecting MERS-CoV sub-genomic mRNA compared with genomic RNA in diverse respiratory specimens | ||
| 520 | |a METHODS: Upper and lower respiratory samples were obtained from 17 MERS-CoV-infected patients. MERS-CoV sub-genomic mRNA was detected by reverse transcription PCR (RT-PCR) and MERS-CoV genomic RNA by real-time RT-PCR | ||
| 520 | |a RESULTS: In sputum and transtracheal aspirate, sub-genomic mRNA was detected for up to 4 weeks after symptoms developed, which correlated with the detection of genomic RNA. In oropharyngeal and nasopharyngeal swab specimens, the detection of sub-genomic mRNA and genomic RNA did not correlate | ||
| 520 | |a CONCLUSIONS: These findings suggest that MERS-CoV does not replicate well in the upper respiratory tract | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Coronavirus infections | |
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| 700 | 1 | |a Choi, Su-Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Choe, Pyoeng Gyun |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Kyoung-Ho |e verfasserin |4 aut | |
| 700 | 1 | |a Bang, Ji Hwan |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Eu Suk |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Hong Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Sang Won |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Nam Joong |e verfasserin |4 aut | |
| 700 | 1 | |a Peiris, Malik |e verfasserin |4 aut | |
| 700 | 1 | |a Oh, Myoung-Don |e verfasserin |4 aut | |
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