DPP9 enzymatic activity in hematopoietic cells is dispensable for mouse hematopoiesis
Copyright © 2018 European Federation of Immunological Societies. All rights reserved..
Dipeptidyl peptidase 9 (DPP9) is a ubiquitously expressed intracellular prolyl peptidase implicated in immunoregulation. However, its physiological relevance in the immune system remains largely unknown. We investigated the role of DPP9 enzyme in immune system by characterizing DPP9 knock-in mice expressing a catalytically inactive S729A mutant of DPP9 enzyme (DPP9ki/ki mice). DPP9ki/ki mice show reduced number of lymphoid and myeloid cells in fetal liver and postnatal blood but their hematopoietic cells are fully functional and able to reconstitute lymphoid and myeloid lineages even in competitive mixed chimeras. These studies demonstrate that inactivation of DPP9 enzymatic activity does not lead to any perturbations in mouse hematopoiesis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:198 |
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| Enthalten in: |
Immunology letters - 198(2018) vom: 30. Juni, Seite 60-65 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kim, Munkyung [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 03.05.2019 Date Revised 03.05.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.imlet.2018.04.008 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM283569794 |
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| 245 | 1 | 0 | |a DPP9 enzymatic activity in hematopoietic cells is dispensable for mouse hematopoiesis |
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| 500 | |a Date Revised 03.05.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 European Federation of Immunological Societies. All rights reserved. | ||
| 520 | |a Dipeptidyl peptidase 9 (DPP9) is a ubiquitously expressed intracellular prolyl peptidase implicated in immunoregulation. However, its physiological relevance in the immune system remains largely unknown. We investigated the role of DPP9 enzyme in immune system by characterizing DPP9 knock-in mice expressing a catalytically inactive S729A mutant of DPP9 enzyme (DPP9ki/ki mice). DPP9ki/ki mice show reduced number of lymphoid and myeloid cells in fetal liver and postnatal blood but their hematopoietic cells are fully functional and able to reconstitute lymphoid and myeloid lineages even in competitive mixed chimeras. These studies demonstrate that inactivation of DPP9 enzymatic activity does not lead to any perturbations in mouse hematopoiesis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Competitive reconstitution assay | |
| 650 | 4 | |a DPP9 | |
| 650 | 4 | |a DPP9 knock-in enzyme inactive mice | |
| 650 | 4 | |a Dipeptidyl peptidase | |
| 650 | 4 | |a Hematopoiesis | |
| 650 | 4 | |a Hematopoietic stem cells | |
| 650 | 7 | |a Dipeptidyl-Peptidases and Tripeptidyl-Peptidases |2 NLM | |
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| 650 | 7 | |a dipeptidyl peptidase 9, mouse |2 NLM | |
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| 700 | 1 | |a von Muenchow, Lilly |e verfasserin |4 aut | |
| 700 | 1 | |a Le Meur, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Kueng, Benjamin |e verfasserin |4 aut | |
| 700 | 1 | |a Gapp, Berangere |e verfasserin |4 aut | |
| 700 | 1 | |a Weber, Delphine |e verfasserin |4 aut | |
| 700 | 1 | |a Dietrich, William |e verfasserin |4 aut | |
| 700 | 1 | |a Kovarik, Jiri |e verfasserin |4 aut | |
| 700 | 1 | |a Rolink, Antonius G |e verfasserin |4 aut | |
| 700 | 1 | |a Ksiazek, Iwona |e verfasserin |4 aut | |
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