Medaka as a model for ECG analysis and the effect of verapamil
Copyright © 2018 The Authors. Production and hosting by Elsevier B.V. All rights reserved..
The heart of the medaka, a small fish native to East Asia, has electrophysiological aspects similar to mammalian hearts. We found that the heart rates of medaka were more similar to humans than mice or rats. Medaka exhibited similar electrocardiogram patterns to those of humans, suggesting a similarity in cardiac impulse formation and propagation. Their hearts also exhibited similar responsiveness to verapamil, a calcium channel antagonist; atropine, a parasympathetic nerve blocker; propranolol, a sympathetic β-adrenergic blocker; and isoproterenol, a sympathetic β-adrenergic agonist. We successfully analyzed action potentials and cardiac contractile forces in vivo. Verapamil affected action potential duration and reduced heart rate, suggesting the importance of voltage-dependent calcium channels in determining the heart rhythm of medaka. We also analyzed the expression of the voltage-dependent calcium channel β2 subunit, which participates in channel formation in cardiac myocytes, and found that splice variant type-2 was the only major transcript in the heart. Our results indicate that medaka could be an appropriate animal model for studying cardiovascular pharmacology.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2018 |
|---|---|
| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:137 |
|---|---|
| Enthalten in: |
Journal of pharmacological sciences - 137(2018), 1 vom: 01. Mai, Seite 55-60 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yonekura, Manabu [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
CJ0O37KU29 |
|---|
| Anmerkungen: |
Date Completed 05.09.2018 Date Revised 05.09.2018 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jphs.2018.04.003 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM283511648 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM283511648 | ||
| 003 | DE-627 | ||
| 005 | 20231225041045.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jphs.2018.04.003 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0945.xml |
| 035 | |a (DE-627)NLM283511648 | ||
| 035 | |a (NLM)29703643 | ||
| 035 | |a (PII)S1347-8613(18)30068-9 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Yonekura, Manabu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Medaka as a model for ECG analysis and the effect of verapamil |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 05.09.2018 | ||
| 500 | |a Date Revised 05.09.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 The Authors. Production and hosting by Elsevier B.V. All rights reserved. | ||
| 520 | |a The heart of the medaka, a small fish native to East Asia, has electrophysiological aspects similar to mammalian hearts. We found that the heart rates of medaka were more similar to humans than mice or rats. Medaka exhibited similar electrocardiogram patterns to those of humans, suggesting a similarity in cardiac impulse formation and propagation. Their hearts also exhibited similar responsiveness to verapamil, a calcium channel antagonist; atropine, a parasympathetic nerve blocker; propranolol, a sympathetic β-adrenergic blocker; and isoproterenol, a sympathetic β-adrenergic agonist. We successfully analyzed action potentials and cardiac contractile forces in vivo. Verapamil affected action potential duration and reduced heart rate, suggesting the importance of voltage-dependent calcium channels in determining the heart rhythm of medaka. We also analyzed the expression of the voltage-dependent calcium channel β2 subunit, which participates in channel formation in cardiac myocytes, and found that splice variant type-2 was the only major transcript in the heart. Our results indicate that medaka could be an appropriate animal model for studying cardiovascular pharmacology | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Calcium Channel Blockers |2 NLM | |
| 650 | 7 | |a Calcium Channels |2 NLM | |
| 650 | 7 | |a Verapamil |2 NLM | |
| 650 | 7 | |a CJ0O37KU29 |2 NLM | |
| 700 | 1 | |a Kondoh, Nami |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Chong |e verfasserin |4 aut | |
| 700 | 1 | |a Toyama, Yuichi |e verfasserin |4 aut | |
| 700 | 1 | |a Ohba, Takayoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Ono, Kyoichi |e verfasserin |4 aut | |
| 700 | 1 | |a Itagaki, Shirou |e verfasserin |4 aut | |
| 700 | 1 | |a Tomita, Hirofumi |e verfasserin |4 aut | |
| 700 | 1 | |a Murakami, Manabu |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of pharmacological sciences |d 2003 |g 137(2018), 1 vom: 01. Mai, Seite 55-60 |w (DE-627)NLM124584721 |x 1347-8648 |7 nnns |
| 773 | 1 | 8 | |g volume:137 |g year:2018 |g number:1 |g day:01 |g month:05 |g pages:55-60 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jphs.2018.04.003 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 137 |j 2018 |e 1 |b 01 |c 05 |h 55-60 | ||